Document Detail

High-LET radiation enhanced apoptosis but not necrosis regardless of p53 status.
MedLine Citation:
PMID:  15380596     Owner:  NLM     Status:  MEDLINE    
PURPOSE: We analyzed the death pattern of human lung cancer cells harboring different p53 statuses after irradiation with different levels of linear energy transfer (LET). METHODS AND MATERIALS: We used three kinds of human lung cancer cell lines with identical genotypes, except for the p53 gene. These cells were exposed to X-rays or accelerated carbon-ion beams. The cellular sensitivities were determined by a colony-forming assay. The detection and quantification of cell death (apoptosis and necrosis) were evaluated and compared by acridine orange/ethidium bromide double staining for fluorescence microscopy. RESULTS: We found that (1) there was no significant difference in cellular sensitivity to LET radiation >70 KeV/microm, although wild-type p53 cell sensitivity to X-rays was higher than that of mutated p53 or p53-null cells; (2) low-LET radiation effectively induced apoptosis in wild-type p53 cells as compared with mutated p53 and p53-null cells; and (3) high-LET radiation induced p53-independent apoptosis. CONCLUSIONS: Our findings suggest that high-LET radiotherapy is expected to be a valid application for patients carrying mutated p53 cancer cells. We proposed that the elucidation of the p53-independent apoptosis-related genes might provide new insights into radiotherapy for cancer.
Akihisa Takahashi; Hideki Matsumoto; Kazue Yuki; Jun-Ichi Yasumoto; Atsuhisa Kajiwara; Mizuho Aoki; Yoshiya Furusawa; Ken Ohnishi; Takeo Ohnishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation oncology, biology, physics     Volume:  60     ISSN:  0360-3016     ISO Abbreviation:  Int. J. Radiat. Oncol. Biol. Phys.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-21     Completed Date:  2004-11-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7603616     Medline TA:  Int J Radiat Oncol Biol Phys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  591-7     Citation Subset:  IM    
Department of Biology, Nara Medical University, Nara, Japan.
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MeSH Terms
Apoptosis / genetics,  radiation effects*
Carcinoma, Non-Small-Cell Lung / genetics,  radiotherapy
Cell Survival / radiation effects
Genes, p53*
Linear Energy Transfer*
Lung Neoplasms / genetics*,  radiotherapy*
Radiation Tolerance / genetics
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured / radiation effects
Tumor Stem Cell Assay

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