| High-LET radiation enhanced apoptosis but not necrosis regardless of p53 status. | |
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MedLine Citation:
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PMID: 15380596 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: We analyzed the death pattern of human lung cancer cells harboring different p53 statuses after irradiation with different levels of linear energy transfer (LET). METHODS AND MATERIALS: We used three kinds of human lung cancer cell lines with identical genotypes, except for the p53 gene. These cells were exposed to X-rays or accelerated carbon-ion beams. The cellular sensitivities were determined by a colony-forming assay. The detection and quantification of cell death (apoptosis and necrosis) were evaluated and compared by acridine orange/ethidium bromide double staining for fluorescence microscopy. RESULTS: We found that (1) there was no significant difference in cellular sensitivity to LET radiation >70 KeV/microm, although wild-type p53 cell sensitivity to X-rays was higher than that of mutated p53 or p53-null cells; (2) low-LET radiation effectively induced apoptosis in wild-type p53 cells as compared with mutated p53 and p53-null cells; and (3) high-LET radiation induced p53-independent apoptosis. CONCLUSIONS: Our findings suggest that high-LET radiotherapy is expected to be a valid application for patients carrying mutated p53 cancer cells. We proposed that the elucidation of the p53-independent apoptosis-related genes might provide new insights into radiotherapy for cancer. |
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Authors:
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Akihisa Takahashi; Hideki Matsumoto; Kazue Yuki; Jun-Ichi Yasumoto; Atsuhisa Kajiwara; Mizuho Aoki; Yoshiya Furusawa; Ken Ohnishi; Takeo Ohnishi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of radiation oncology, biology, physics Volume: 60 ISSN: 0360-3016 ISO Abbreviation: Int. J. Radiat. Oncol. Biol. Phys. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-21 Completed Date: 2004-11-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7603616 Medline TA: Int J Radiat Oncol Biol Phys Country: United States |
Other Details:
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Languages: eng Pagination: 591-7 Citation Subset: IM |
Affiliation:
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Department of Biology, Nara Medical University, Nara, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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genetics,
radiation effects* Carcinoma, Non-Small-Cell Lung / genetics, radiotherapy Cell Survival / radiation effects Genes, p53* Humans Linear Energy Transfer* Lung Neoplasms / genetics*, radiotherapy* Necrosis Radiation Tolerance / genetics Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured / radiation effects Tumor Stem Cell Assay |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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