| High HIV-1 genetic diversity in Cuba. | |
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MedLine Citation:
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PMID: 12172086 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: HIV-1 subtype B is largely predominant in the Caribbean, although other subtypes have been recently identified in Cuba. OBJECTIVES: To examine HIV-1 genetic diversity in Cuba. METHODS: The study enrolled 105 HIV-1-infected individuals, 93 of whom had acquired the infection in Cuba. DNA from peripheral blood mononuclear cells was used for polymerase chain reaction amplification and sequencing of pol (protease-reverse transcriptase) and env (V3 region) segments. Phylogenetic trees were constructed using the neighbour-joining method. Intersubtype recombination was analysed by bootscanning. RESULTS: Of the samples, 50 (48%) were of subtype B and 55 (52%) of diverse non-B subtypes and recombinant forms. Among non-B viruses, 12 were non-recombinant, belonging to six subtypes (C, D, F1, G, H and J), the most frequent of which was subtype G (n = 5). The remaining 43 (78%) non-B viruses were recombinant, with 14 different forms, the two most common of which were Dpol/Aenv (n = 21) and U(unknown)pol/Henv (n = 7), which grouped in respective monophyletic clusters. Twelve recombinant viruses were mosaics of different genetic forms circulating in Cuba. Overall, 21 genetic forms were identified, with all known HIV-1 group M subtypes present in Cuba, either as non-recombinant viruses or as segments of recombinant forms. Non-B subtype viruses were predominant among heterosexuals (72%) and B subtype viruses among homo- or bisexuals (63%). CONCLUSION: An extraordinarily high diversity of HIV-1 genetic forms, unparalleled in the Americas and comparable to that found in Central Africa, is present in Cuba. |
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Authors:
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María Teresa Cuevas; Ignacio Ruibal; María Luisa Villahermosa; Héctor Díaz; Elena Delgado; Elena Vázquez-de Parga; Lucía Pérez-Alvarez; Madelín Blanco de Armas; Laureano Cuevas; Leandro Medrano; Enrique Noa; Saladin Osmanov; Rafael Nájera; Michael M Thomson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: AIDS (London, England) Volume: 16 ISSN: 0269-9370 ISO Abbreviation: AIDS Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-12 Completed Date: 2002-10-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8710219 Medline TA: AIDS Country: England |
Other Details:
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Languages: eng Pagination: 1643-53 Citation Subset: IM; X |
Affiliation:
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Instituto de Salud Carlos III, Madrid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cuba
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epidemiology Databases, Genetic Female Genes, pol Genetic Variation* HIV Envelope Protein gp120 / genetics HIV Infections / epidemiology, virology* HIV-1 / classification, genetics* Humans Male Peptide Fragments / genetics Phylogeny |
| Chemical | |
Reg. No./Substance:
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0/HIV Envelope Protein gp120; 0/HIV envelope protein gp120 (305-321); 0/Peptide Fragments |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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