Document Detail


High Frequency of Glucose Utilizing Mutants in Shewanella oneidensis MR-1.
MedLine Citation:
PMID:  22092702     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Shewanella oneidensis MR-1 has conventionally been considered unable to use glucose as a carbon substrate for growth. The genome sequence of S. oneidensis MR-1 however, suggests the ability to use glucose. Here we demonstrate that during initial glucose exposure, S. oneidensis MR-1 quickly and frequently gains the ability to utilize glucose as a sole carbon source, in contrast to wild-type S. oneidensis which cannot immediately use glucose as a sole carbon substrate. HPLC and (14) C-glucose tracer studies confirm the disappearance in cultures and assimilation and respiration, respectively, of glucose. The relatively short time frame with which S. oneidensis MR-1 gained the ability to use glucose raises interesting ecological implications.
Authors:
Erinn C Howard; Leila J Hamdan; Stephen E Lizewski; Bradley R Ringeisen
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-5
Journal Detail:
Title:  FEMS microbiology letters     Volume:  -     ISSN:  1574-6968     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
National Research Council, Washington, D.C.; Bioenergy and Biofabrication Section, Code 6115.
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