| A high-fat diet elicits differential responses in genes coordinating oxidative metabolism in skeletal muscle of lean and obese individuals. | |
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MedLine Citation:
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PMID: 21190973 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CONTEXT: In lean individuals, increasing dietary lipid can elicit an increase in whole body lipid oxidation; however, with obesity the capacity to respond to changes in substrate availability appears to be compromised. OBJECTIVE: To determine whether the responses of genes regulating lipid oxidation in skeletal muscle differed between lean and insulin resistant obese humans upon exposure to a high-fat diet (HFD). DESIGN AND SETTING: A 5-d prospective study conducted in the research unit of an academic center. PARTICIPANTS: Healthy, lean (n = 12; body mass index = 22.1 ± 0.6 kg/m(2)), and obese (n=10; body mass index = 39.6 ± 1.7 kg/m(2)) males and females, between ages 18 and 30. INTERVENTION: Participants were studied before and after a 5-d HFD (65% fat). MAIN OUTCOME MEASURES: Skeletal muscle biopsies (vastus lateralis) were obtained in the fasted and fed states before and after the HFD and mRNA content for genes involved with lipid oxidation determined. Skeletal muscle acylcarnitine content was determined in the fed states before and after the HFD. RESULTS: Peroxisome proliferator activated receptor (PPAR) α mRNA content increased in lean, but not obese, subjects after a single high-fat meal. From Pre- to Post-HFD, mRNA content exhibited a body size × HFD interaction, where the lean individuals increased while the obese individuals decreased mRNA content for pyruvate dehydrogenase kinase 4, uncoupling protein 3, PPARα, and PPARγ coactivator-1α (P ≤ 0.05). In the obese subjects medium-chain acylcarnitine species tended to accumulate, whereas no change or a reduction was evident in the lean individuals. CONCLUSIONS: These findings indicate a differential response to a lipid stimulus in the skeletal muscle of lean and insulin resistant obese humans. |
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Authors:
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K E Boyle; J P Canham; L A Consitt; D Zheng; T R Koves; T P Gavin; D Holbert; P D Neufer; O Ilkayeva; D M Muoio; J A Houmard |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-12-29 |
Journal Detail:
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Title: The Journal of clinical endocrinology and metabolism Volume: 96 ISSN: 1945-7197 ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-07 Completed Date: 2011-05-23 Revised Date: 2012-03-01 |
Medline Journal Info:
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Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: 775-81 Citation Subset: AIM; IM |
Affiliation:
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Human Performance Laboratory, East Carolina University, Greenville, North Carolina 27858, USA. kristen.boyle@ucdenver.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Blood Glucose / metabolism Body Mass Index Carnitine / analogs & derivatives, metabolism Diet Dietary Fats / pharmacology* Fatty Acids, Nonesterified / blood Female Gene Expression Regulation / drug effects Humans Insulin / blood, metabolism Ion Channels / genetics, metabolism Lipid Metabolism / drug effects, genetics* Male Mitochondrial Proteins / genetics, metabolism Muscle, Skeletal / chemistry, metabolism* Obesity / metabolism* Oxidation-Reduction PPAR alpha / biosynthesis, genetics Pyruvate Dehydrogenase (Lipoamide) / genetics, metabolism RNA, Messenger / biosynthesis, genetics Spectrometry, Mass, Electrospray Ionization Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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AG025205/AG/NIA NIH HHS; DK56112/DK/NIDDK NIH HHS; R01 DK074825-02/DK/NIDDK NIH HHS; R01 DK074825-03/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Dietary Fats; 0/Fatty Acids, Nonesterified; 0/Insulin; 0/Ion Channels; 0/Mitochondrial Proteins; 0/PPAR alpha; 0/RNA, Messenger; 0/acylcarnitine; 0/mitochondrial uncoupling protein 3; 541-15-1/Carnitine; EC 1.2.4.1/Pyruvate Dehydrogenase (Lipoamide) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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