Document Detail


High DNA content and prognosis in lymph node positive breast cancer. A case control study by the University of Leiden and ECOG. (Eastern Cooperative Oncology Group).
MedLine Citation:
PMID:  8123866     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate whether breast cancer cells with unusually high nuclear DNA content are associated with an adverse outcome, Eastern Cooperative Oncology Group investigators selected breast cancer trial patients who suffered an early death (ED) within two years after diagnosis to compare with other trial patients who had a survival of at least 7.5 years. Paraffin blocks of primary breast cancers were obtained from 93 evaluable patients who had been enrolled in two surgical adjuvant trials for lymph node positive (LN+) disease (T1-3N1M0). Single cell monolayer preparations from these blocks were stained with acriflavine-Feulgen and analyzed by image analysis for DNA content with the automated Leiden Television Analysis System (LEY-TAS). Standard prognostic variables (estrogen receptor (ER) status, number of lymph nodes with metastases, and size of the cancer) were compared with three DNA content characteristics: DNA ploidy status, number of nuclei with > 5C DNA content, and percent of nuclei with > 5 C. Estimates of the odds ratio in multivariate comparisons showed that ER negativity was associated with ED (p = 0.0005) and an odds ratio estimate using negative/positive of 4.87. The number of positive lymph nodes associated with ED had a p-value of 0.0005 and an odds ratio estimate of 4.63 when comparing the > 3 nodes group to the 1-3 nodes group. In contrast, the strongest association for any of the DNA content characteristics with ED had a p-value of 0.017 and an odds ratio estimate of 2.76. This power of association disappeared when stratified on ER status.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
K W Gilchrist; R Gray; A M van Driel-Kulker; W E Mesker; J J Ploem-Zaaijer; J S Ploem; S G Taylor; D C Tormey
Related Documents :
18369636 - The role of lymphadenectomy in high risk prostate cancer.
22252966 - Associations among physical activity, body mass index, and health-related quality of li...
8443766 - How to integrate steroid hormone receptor, flow cytometric, and other prognostic inform...
3536526 - Comparison of halsted mastectomy with quadrantectomy, axillary dissection, and radiothe...
12131306 - Hormone therapy for locally advanced prostate cancer.
24986326 - Nf-κb signaling inhibition and anticancer activities of lldt-246 on human colorectal ca...
Publication Detail:
Type:  Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Breast cancer research and treatment     Volume:  28     ISSN:  0167-6806     ISO Abbreviation:  Breast Cancer Res. Treat.     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1994-04-14     Completed Date:  1994-04-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8111104     Medline TA:  Breast Cancer Res Treat     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Affiliation:
Wisconsin Comprehensive Cancer Center, Madison, CA 21076.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aged
Aneuploidy*
Breast Neoplasms / epidemiology*,  genetics*,  mortality
Case-Control Studies
Cell Nucleus / metabolism
Clinical Trials as Topic
DNA, Neoplasm / genetics*,  metabolism
Female
Flow Cytometry
Humans
Image Processing, Computer-Assisted
Lymphatic Metastasis
Prognosis
Prospective Studies
Television
Grant Support
ID/Acronym/Agency:
CA 21115/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Improved outcome for high-risk acute myeloid leukemia patients using autologous bone marrow transpla...
Next Document:  Progesterone induction of mammary carcinomas in BALB/c female mice. Correlation between progestin de...