Document Detail

High DNA content and prognosis in lymph node positive breast cancer. A case control study by the University of Leiden and ECOG. (Eastern Cooperative Oncology Group).
MedLine Citation:
PMID:  8123866     Owner:  NLM     Status:  MEDLINE    
To investigate whether breast cancer cells with unusually high nuclear DNA content are associated with an adverse outcome, Eastern Cooperative Oncology Group investigators selected breast cancer trial patients who suffered an early death (ED) within two years after diagnosis to compare with other trial patients who had a survival of at least 7.5 years. Paraffin blocks of primary breast cancers were obtained from 93 evaluable patients who had been enrolled in two surgical adjuvant trials for lymph node positive (LN+) disease (T1-3N1M0). Single cell monolayer preparations from these blocks were stained with acriflavine-Feulgen and analyzed by image analysis for DNA content with the automated Leiden Television Analysis System (LEY-TAS). Standard prognostic variables (estrogen receptor (ER) status, number of lymph nodes with metastases, and size of the cancer) were compared with three DNA content characteristics: DNA ploidy status, number of nuclei with > 5C DNA content, and percent of nuclei with > 5 C. Estimates of the odds ratio in multivariate comparisons showed that ER negativity was associated with ED (p = 0.0005) and an odds ratio estimate using negative/positive of 4.87. The number of positive lymph nodes associated with ED had a p-value of 0.0005 and an odds ratio estimate of 4.63 when comparing the > 3 nodes group to the 1-3 nodes group. In contrast, the strongest association for any of the DNA content characteristics with ED had a p-value of 0.017 and an odds ratio estimate of 2.76. This power of association disappeared when stratified on ER status.(ABSTRACT TRUNCATED AT 250 WORDS)
K W Gilchrist; R Gray; A M van Driel-Kulker; W E Mesker; J J Ploem-Zaaijer; J S Ploem; S G Taylor; D C Tormey
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Publication Detail:
Type:  Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Breast cancer research and treatment     Volume:  28     ISSN:  0167-6806     ISO Abbreviation:  Breast Cancer Res. Treat.     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1994-04-14     Completed Date:  1994-04-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8111104     Medline TA:  Breast Cancer Res Treat     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Wisconsin Comprehensive Cancer Center, Madison, CA 21076.
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MeSH Terms
Breast Neoplasms / epidemiology*,  genetics*,  mortality
Case-Control Studies
Cell Nucleus / metabolism
Clinical Trials as Topic
DNA, Neoplasm / genetics*,  metabolism
Flow Cytometry
Image Processing, Computer-Assisted
Lymphatic Metastasis
Prospective Studies
Grant Support
Reg. No./Substance:
0/DNA, Neoplasm

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