| High-Content Imaging in Cervical Cancer Screening. | |
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MedLine Citation:
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PMID: 22972847 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A shift from conventional cytology to a molecular approach could improve cervical cancer screening. This proof-of-concept study aims to develop a high-content imaging platform for the simultaneous detection of multiple biomarkers for cervical disease. Liquid-based cytology (LBC) samples were used to optimize a dual ProExC/Ki-67 immunofluorescence staining protocol for SurePath-fixed cells. The simultaneous and automated detection of these biomarkers was performed using the BD Pathway 435 system. The ability of high-content imaging to detect dysplastic cervical cells was assessed using keratinocytes spiked with immunopositive SiHa cells and a high-grade squamous intraepithelial lesion (HSIL) LBC sample. The percentages of Ki-67- and ProExC-immunopositive objects correlated significantly with the percentages of spiked SiHa cells. The dysplastic cells of the HSIL sample could be detected using high-content cell analysis. In conclusion, high-content imaging allows the simultaneous and automated detection of Ki-67- and ProExC-immunopositive dysplastic cells in LBC specimens. |
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Authors:
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Maria Isabel Micalessi; Gaëlle Boulet; Isabel Pintelon; Peter Verstraelen; Frans Nauwelaers; Martin Ryser; Johannes Bogers |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-12 |
Journal Detail:
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Title: Journal of biomolecular screening Volume: - ISSN: 1552-454X ISO Abbreviation: J Biomol Screen Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9612112 Medline TA: J Biomol Screen Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Applied Molecular Biology Research (AMBIOR) Group, Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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