Document Detail

A hierarchical response of cells to perpendicular micro- and nanometric textural cues.
MedLine Citation:
PMID:  19278933     Owner:  NLM     Status:  MEDLINE    
In this paper, we report on the influence of shallow micro- and nanopatterned substrata on the attachment and behavior of a human fibroblast [human telomerase transfected immortalized (hTERT)] cells. We identify a hierarchy of textural guidance cues with respect to cell alignment on these substrates. Cells were seeded and cultured for 48 h on silicon substrates patterned with two linear textures overlaid at 90 degrees, both with 24 microm pitch: a micrograting and a nanopattern of rows of 140- nm-diameter pits arranged in a rectangular array with 300 nm centre-to-centre spacing. The cell response to these textures was shown to be highly dependent on textural feature dimensions. We show that cells align to the stripes of nanopits. Stripes of 30-nm deep nanopits were also shown to elicit a stronger response from cells than 160-nm deep nanopits.
K Seunarine; Adam S G Curtis; D O Meredith; Chris D W Wilkinson; M O Riehle; N Gadegaard
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Publication Detail:
Type:  Journal Article     Date:  2009-03-10
Journal Detail:
Title:  IEEE transactions on nanobioscience     Volume:  8     ISSN:  1558-2639     ISO Abbreviation:  IEEE Trans Nanobioscience     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2010-01-06     Completed Date:  2010-03-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101152869     Medline TA:  IEEE Trans Nanobioscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  219-25     Citation Subset:  IM    
University of Glasgow, Centre for Cell Engineering, Glasgow G12 8QQ, U.K.
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MeSH Terms
Cell Adhesion
Cell Culture Techniques / methods*
Cell Line
Cell Polarity
Crystallization / methods
Fibroblasts / cytology*,  physiology*
Materials Testing
Mechanotransduction, Cellular / physiology*
Molecular Conformation
Nanostructures / chemistry*,  ultrastructure*
Nanotechnology / methods
Particle Size
Surface Properties
Tissue Engineering / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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