| Hexose transport across the apical and basolateral membrane of enterocytes from different regions of the chicken intestine. | |
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MedLine Citation:
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PMID: 8146029 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The properties of hexose transport across the apical and basolateral membranes of chicken enterocytes have been studied in the small and large intestine. Results show that (a) isolated epithelial cells from all segments except the coprodeum can accumulate 3-O-methylglucose (Glc3Me) against a concentration gradient, by a Na(+)-dependent and phloridizin-sensitive mechanism. (b) The cell cumulative capacity for Glc3Me (control/phloridizin-incubated cells) is lower in the small intestine than in the large intestine (rectum = proximal caecum = ileum > jejunum > duodenum). (c) Theophylline enhances the cell Glc3Me cumulative capacity 2.9-fold in the duodenum and 2.4-fold in the jejunum but has no effect in the other segments studied. (d) Analysis of sugar uptake indicates that net hexose influx rates decrease from proximal to distal regions: jejunum > duodenum > ileum = proximal caecum = rectum for the apical transport system (alpha-methyl glucoside as substrate and phloridizin as inhibitor) and duodenum > jejunum > ileum = proximal caecum = rectum for the basolateral system (2-deoxyglucose; theophylline). (e) The duodenum and the jejunum show high apical and basolateral hexose transport rates, which confer a significant capacity for sugar absorption on the proximal intestine. More distal regions, including the ileum, the proximal caecum and the rectum, have transport systems analogous to those of the proximal intestine that keep a considerable potential capability to recover hexoses from the lumen. |
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Authors:
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R Ferrer; M Gil; M Moretó; M Oliveras; J M Planas |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 426 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-05-03 Completed Date: 1994-05-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: GERMANY |
Other Details:
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Languages: eng Pagination: 83-8 Citation Subset: IM |
Affiliation:
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Department de Ciències Fisiològiques Humanes i de la Nutrició, Universitat de Barcelona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3-O-Methylglucose Animals Biological Transport, Active / drug effects Chickens Deoxyglucose / pharmacokinetics Epithelium / drug effects, metabolism Hexoses / pharmacokinetics* Intestinal Absorption / drug effects Intestines / drug effects, metabolism* Male Methylglucosides / pharmacokinetics Theophylline / pharmacology Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Hexoses; 0/Methylglucosides; 146-72-5/3-O-Methylglucose; 154-17-6/Deoxyglucose; 25360-07-0/methylglucoside; 58-55-9/Theophylline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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