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Hexavalent chromium disrupts mitosis by stabilizing microtubules in Lens culinarisroot tip cells.
MedLine Citation:
PMID:  22607451     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Hexavalent chromium [Cr(VI)] is an accumulating environmental pollutant due to anthropogenic activities, toxic for humans, animals and plants. Therefore, the effects of Cr(VI) on dividing root cells of lentil (Lens culinaris) were investigated by tubulin immunofluorescence and DNA staining. In Cr(VI)-treated roots, cell divisions wereperturbed, thechromosomes formed irregular aggregations, multinucleate cells were produced andtubulin clusterswere entrappedwithin the nuclei. All cell cycle-specific microtubule(MT) arrayswereaffected, indicating a stabilizing effect of Cr(VI) on the MTs of L. culinaris. Besides, a time- and concentration-dependent gradual increase of acetylated a-tubulin, an indicator of MT stabilization,was observed in Cr(VI)-treated roots by both immunofluorescence and Western blotting. Evidence is also provided that reactive oxygen species (ROS) caused by Cr(VI), determined with the specific marker dichlorofluorescein, may be responsible for MT stabilization.Combined treatments with Cr(VI) and oryzalin revealed that Cr(VI) overcomes the depolymerizing ability of oryzalin, as it does experimentally introduced hydrogen peroxide, further supporting its stabilizing effect.In conclusion, it is suggested that the mitotic aberrations caused by Cr(VI)in L. culinaris root cells may be the result of MT stabilization rather thandepolymerization, which consequently disturbs MT dynamics and their related functions.
Authors:
Eleftherios P Eleftheriou; Ioannis-Dimosthenis S Adamakis; Maria Fatsiou; Emmanuel Panteris
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-18
Journal Detail:
Title:  Physiologia plantarum     Volume:  9999     ISSN:  1399-3054     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1256322     Medline TA:  Physiol Plant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © Physiologia Plantarum 2012.
Affiliation:
Department of Botany, School of Biology, Aristotle University of Thessaloniki, GR-541 24, Thessaloniki, Greece.
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