Document Detail


Hexaploid H1 (ES) cells established from octaploid H1 cells are as DNA stable as pentaploid H1 cells.
MedLine Citation:
PMID:  21547691     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hexaploid H1 (ES) cells (6H1 cells) were established from octaploid H1 cells (8H1 cells), as were pentaploid H1 cells (5H1 cells). 6H1 cells were compared with 5H1 cells. The number of chromosomes of 6H1 cells was 115, 20 more than the 95 of 5H1 cells. The durations of G(1), S, and G(2)/M phases of 6H1 cells were 3, 7, and 6 h, respectively, almost the same as those of 5H1 cells. The cell volume of 6H1 cells was equivalent that of 5H1 cells. The morphology of 6H1 cells was flattened circular cluster, different from the spherical cluster of 5H1 cells. 6H1 cells exhibited alkaline phosphatase activity as well as 5H1 cells. The DNA content of 6H1 cells was stable and maintained for 300 days of culturing, the same as that of 5H1 cells. The DNA stability of 6H1 cells was explained using a hypothesis concerning the DNA structure of polyploid cells because the asymmetric configuration of homologous chromosomes in 6H1 cells inhibited chromosome loss.
Authors:
Kohzaburo Fujikawa-Yamamoto; Minoru Miyagoshi; Xianwen Luo; Hiroko Yamagishi
Publication Detail:
Type:  Journal Article     Date:  2010-12-29
Journal Detail:
Title:  Human cell     Volume:  24     ISSN:  1749-0774     ISO Abbreviation:  Hum. Cell     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-05-06     Completed Date:  2011-09-14     Revised Date:  2012-05-31    
Medline Journal Info:
Nlm Unique ID:  8912329     Medline TA:  Hum Cell     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  13-20     Citation Subset:  IM    
Affiliation:
Division of Cell Medicine, Research Institute of Medical Science, Kanazawa Medical University, Uchinada, Ishikawa, Japan. fujikawa@kanazawa-med.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / analysis
Animals
Cell Size
Cells, Cultured
Chromosomes*
Cloning, Organism
DNA / physiology*
Embryonic Stem Cells / cytology*
Mice
Polyploidy*
Chemical
Reg. No./Substance:
9007-49-2/DNA; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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