| Hexane extract of Raphanus sativus L. roots inhibits cell proliferation and induces apoptosis in human cancer cells by modulating genes related to apoptotic pathway. | |
| | |
MedLine Citation:
|
PMID: 20652750 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Raphanus sativus, a common cruciferous vegetable has been attributed to possess a number of pharmacological and therapeutic properties. It has been used in indigenous system of medicine for the treatment of various human ailments in India. This present study evaluated the chemopreventive efficacy of different parts of R. sativus such as root, stem and leaves, extracted with solvents of varying polarity and investigated the molecular mechanism leading to growth arrest and apoptotic cell death in human cancer cell lines. Of the different parts, significant growth inhibitory effect was observed with hexane extract of R. sativus root. Analysis of hexane extract by GC-MS revealed the presence of several isothiocyanates (ITCs) such as 4-(methylthio)-3-butenyl isothiocyanate (MTBITC), 4-(methylthio)-3-butyl isothiocyanate (erucin), 4-methylpentyl isothiocyanate, 4-pentenyl isothiocyanate and sulforaphene. R. sativus root extract induced cell death both in p53 proficient and p53 deficient cell lines through induction of apoptotic signaling pathway regardless of the p53 status of cells. The molecular mechanisms underlying R. sativus-induced apoptosis may involve interactions among Bcl(2) family genes, as evidenced by up-regulation of pro-apoptotic genes and down-regulation of anti-apoptotic genes along with activation of Caspase-3. Our findings present the first evidence that hexane extract of R. sativus root exerts potential chemopreventive efficacy and induces apoptosis in cancer cell lines through modulation of genes involved in apoptotic signaling pathway. |
| | |
Authors:
|
Syed Sultan Beevi; Lakshmi Narasu Mangamoori; Murugan Subathra; Jyotheeswara Reddy Edula |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Plant foods for human nutrition (Dordrecht, Netherlands) Volume: 65 ISSN: 1573-9104 ISO Abbreviation: Plant Foods Hum Nutr Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-09-24 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8803554 Medline TA: Plant Foods Hum Nutr Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 200-9 Citation Subset: IM |
Affiliation:
|
Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad 500085, Andhra Pradesh, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antineoplastic Agents, Phytogenic
/
pharmacology*,
therapeutic use Apoptosis / drug effects*, genetics Caspase 3 / genetics, metabolism Cell Line, Tumor Cell Proliferation / drug effects* DNA-Binding Proteins / metabolism Gene Expression Regulation, Neoplastic / drug effects* Hela Cells Humans Isothiocyanates / pharmacology, therapeutic use Neoplasms / drug therapy*, pathology Nuclear Proteins / metabolism Phytotherapy Plant Extracts / pharmacology*, therapeutic use Plant Roots Proto-Oncogene Proteins c-bcl-2 / genetics, metabolism Raphanus / chemistry* Signal Transduction / drug effects Thiocyanates / pharmacology, therapeutic use Tumor Suppressor Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antineoplastic Agents, Phytogenic; 0/DNA-Binding Proteins; 0/Isothiocyanates; 0/Nuclear Proteins; 0/Plant Extracts; 0/Proto-Oncogene Proteins c-bcl-2; 0/Thiocyanates; 0/Tumor Suppressor Proteins; 0/tumor suppressor protein p73; 4478-93-7/sulforafan; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Analysis of athletes' heart by tissue Doppler and strain/strain rate imaging.
Next Document: Doctor-cared dying instead of physician-assisted suicide: a perspective from Germany.