Document Detail

Hexadecylphosphocholine inhibits translocation of CTP:choline-phosphate cytidylyltransferase in Madin-Darby canine kidney cells.
MedLine Citation:
PMID:  1313020     Owner:  NLM     Status:  MEDLINE    
The mechanism of the inhibition of phosphatidylcholine biosynthesis by the phospholipid analogue, hexadecylphosphocholine, was investigated in Madin-Darby canine kidney cells. In the presence of 50 mumol/liter hexadecylphosphocholine, there was a translocation of CTP:choline-phosphate cytidylyltransferase (EC activity from the membranes to the cytosol of the cells. Since we recently demonstrated that hexadecylphosphocholine also inhibits protein kinase C in vitro, [methyl-3H]choline labeling experiments were repeated with phorbol ester-desensitized cells. In these cells the same inhibitory effect of hexadecylphosphocholine was measured. As a consequence of inhibition, the [methyl-3H]choline incorporation into the phosphocholine pool was increased time-dependently. In addition, there was no evidence for a difference between the choline uptake of control and hexadecylphosphocholine-treated cells. Likewise, the amount of diacylglycerol, a known activator of the translocation process, was not reduced. Finally, we showed that the inhibitory effect of hexadecylphosphocholine on CTP:choline-phosphate cytidylyltransferase translocation cannot be explained by the detergent properties of this phospholipid analogue. Therefore, we suggest a direct inhibitory effect of hexadecylphosphocholine on the translocation of CTP:choline-phosphate cytidylyltransferase.
C C Geilen; T Wieder; W Reutter
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  267     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-04-28     Completed Date:  1992-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6719-24     Citation Subset:  IM    
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany.
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MeSH Terms
Biological Transport / drug effects
Cells, Cultured
Choline / metabolism
Choline-Phosphate Cytidylyltransferase
Diglycerides / metabolism
Kidney / cytology,  drug effects*,  enzymology
Nucleotidyltransferases / metabolism*
Phosphatidylcholines / biosynthesis
Phosphorylcholine / analogs & derivatives*,  pharmacology
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Diglycerides; 0/Phosphatidylcholines; 107-73-3/Phosphorylcholine; 16561-29-8/Tetradecanoylphorbol Acetate; 58066-85-6/miltefosine; 62-49-7/Choline; EC 2.7.7.-/Nucleotidyltransferases; EC Cytidylyltransferase

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