Document Detail


Heterotrimeric guanosine triphosphate-binding protein-coupled modulatory actions of motilin on K(+) channels and postsynaptic γ-aminobutyric acid receptors in mouse medial vestibular nuclear neurons.
MedLine Citation:
PMID:  23136934     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Some central nervous system neurons express receptors of gastrointestinal hormones, but their pharmacological actions are not well known. Previous anatomical and unit recording studies suggest that a group of cerebellar Purkinje cells express motilin receptors, and motilin depresses the spike discharges of vestibular nuclear neurons that receive direct cerebellar inhibition in rats or rabbits. Here, by the slice-patch recording method, we examined the pharmacological actions of motilin on the mouse medial vestibular nuclear neurons (MVNs), which play an important role in the control of ocular reflexes. A small number of MVNs, as well as cerebellar floccular Purkinje cells, were labeled with an anti-motilin receptor antibody. Bath application of motilin (0.1 μm) decreased the discharge frequency of spontaneous action potentials in a group of MVNs in a dose-dependent manner (K(d) , 0.03 μm). The motilin action on spontaneous action potentials was blocked by apamin (100 nm), a blocker of small-conductance Ca(2+) -activated K(+) channels. Furthermore, motilin enhanced the amplitudes of inhibitory postsynaptic currents (IPSCs) and miniature IPSCs, but did not affect the frequencies of miniature IPSCs. Intracellular application of pertussis toxin (PTx) (0.5 μg/μL) or guanosine triphosphate-γ-S (1 mm) depressed the motilin actions on both action potentials and IPSCs. Only 30% of MVNs examined on slices obtained from wild-type mice, but none of the GABAergic MVNs that were studied on slices obtained from vesicular γ-aminobutyric acid transporter-Venus transgenic mice, showed such a motilin response on action potentials and IPSCs. These findings suggest that motilin could modulate small-conductance Ca(2+) -activated K(+) channels and postsynaptic γ-aminobutyric acid receptors through heterotrimeric guanosine triphosphate-binding protein-coupled receptor in a group of glutamatergic MVNs.
Authors:
Hiroshi Todaka; Tetsuya Tatsukawa; Tsutomu Hashikawa; Yuchio Yanagawa; Katsuei Shibuki; Soichi Nagao
Related Documents :
16327284 - Haemodynamic and exocrine effects of caerulein at submaximal and supramaximal levels on...
24168364 - Positive allosteric modulators (pams) of metabotropic glutamate receptor 5 (mglur5) att...
24418814 - The ly49 natural killer cell receptors: a versatile tool for viral self-discrimination.
9544204 - Novel nonpeptide cck-b antagonists: design and development of quinazolinone derivatives...
8634374 - Purine suppression of proliferation of sertoli-like tm4 cells in culture.
12144934 - Tyrosine-induced release of dopamine is under inhibitory control of presynaptic dopamin...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-9
Journal Detail:
Title:  The European journal of neuroscience     Volume:  -     ISSN:  1460-9568     ISO Abbreviation:  Eur. J. Neurosci.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8918110     Medline TA:  Eur J Neurosci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Neuroscience Societies and Blackwell Publishing Ltd.
Affiliation:
Laboratory for Motor Learning Control, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako-Shi, Saitama, 351-0198, Japan; Department of Neurophysiology, Brain Research Institute, Niigata University, Niigata, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Design of a Single-Tube, Endpoint, LATE-PCR Assay for 17 Pathogens Associated with Sepsis.
Next Document:  Changes over time in craniocerebral morphology and syringomyelia in cavalier King Charles spaniels w...