Document Detail

Heterotopic hindlimb allotransplantation in rats: an alternative model for immunological research in composite-tissue allotransplantation.
MedLine Citation:
PMID:  16037937     Owner:  NLM     Status:  MEDLINE    
A nonfunctional heterotopic hindlimb allotransplantation model is presented, and a study was performed between this model and standard orthotopic hindlimb transplantation to compare operation and ischemia time, overall morbidity, and mortality rates. In this model, the skin component of the hindlimb was stripped away to the ankle level, and the remaining part along with the vascularized epigastric skin was transplanted heterotopically to the inguinal space of the recipient. No osteotomy and intramedullary fixation were performed. Thirty heterotopic and orthotopic hindlimb transplantations were performed in two groups. Each group involved 15 transplantations from inbred, male Brown Norway (BN; RT1n) weighing 150-200 g to Lewis rats (LEW; RT1(1)) weighing 250-300 g. The animals were followed up for 2 weeks under immunosuppression with cyclosporine A (16 mg/kg/day). Mean operation times for heterotopic and orthotopic hindlimb transplantations were 60 and 105 min, with ischemia times of 35 and 85 min, respectively. No animal deaths or major complications were encountered in heterotopic hindlimb transplantation during the follow-up period. Seroma formation was observed in one animal as the only minor complication. The mortality rate for orthotopic hindlimb transplantation was 26.7%, and there were minor complications in 35%, including infection, ulceration, and loss of rigid fixation. This model circumvents the disadvantages of osteotomy and intramedullary fixation, which may increase the risk of blood loss, embolus, and infection in immunosuppressed animals. It also helps avoid tension or kinking on the anastomosis due to inadequate judgment of the osteotomy level, distortion associated with loss of rigid fixation, and weight mismatch between donor and recipient. From the immunological point of view, insult to bone marrow is avoided, and a relatively constant amount of bone marrow is introduced to be used in chimerism-based tolerance studies. We recommend this model for composite-tissue allotransplantation studies when functional recovery is not of primary importance.
Ali Engin Ulusal; Betul Gozel Ulusal; Li-Man Hung; Fu Chan Wei
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microsurgery     Volume:  25     ISSN:  0738-1085     ISO Abbreviation:  Microsurgery     Publication Date:  2005  
Date Detail:
Created Date:  2005-08-02     Completed Date:  2005-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309230     Medline TA:  Microsurgery     Country:  United States    
Other Details:
Languages:  eng     Pagination:  410-4     Citation Subset:  IM    
Copyright Information:
Copyright 2005 Wiley-Liss, Inc.
Department of Plastic Surgery, Chang Gung Memorial Hospital, Chang Gung Medical College, Chang Gung University, Taipei, Taiwan.
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MeSH Terms
Graft Rejection / immunology
Hindlimb / transplantation*
Immune Tolerance / immunology
Models, Animal
Transplantation Immunology / immunology
Transplantation, Heterotopic / immunology,  methods*
Transplantation, Homologous / immunology,  methods*

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