Document Detail


Heterophilic binding of the adhesion molecules poliovirus receptor and immunoglobulin superfamily 4A in the interaction between mouse spermatogenic and Sertoli cells.
MedLine Citation:
PMID:  17314315     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cell adhesion protein immunoglobulin superfamily 4A (IGSF4A) is expressed on the surfaces of spermatogenic cells in the mouse testis. During spermatogenesis, IGSF4A is considered to bind to the surface of Sertoli cells in a heterophilic manner. To identify this unknown partner of IGSF4A, we generated rat monoclonal antibodies against the membrane proteins of mouse Sertoli cells grown in primary culture. Using these monoclonal antibodies, we isolated a clone that immunostained Sertoli cells and reacted with the product of immunoprecipitation of the homogenate of mouse testis with anti-IGSF4A antibody. Subsequently, to identify the Sertoli cell membrane protein that is recognized by this monoclonal antibody, we performed expression cloning of a cDNA library from the mouse testis. As a result, we identified poliovirus receptor (PVR), which is another IGSF-type cell adhesion molecule, as the binding partner of IGSF4A. The antibodies raised against PVR and IGSF4A immunoprecipitated both antigens in the homogenate of mouse testis. Immunoreactivity for PVR was present in Sertoli cells but not in spermatogenic cells at all stages of spermatogenesis. Overexpression of PVR in TM4, a mouse Sertoli cell line, increased more than three-fold its capacity to adhere to Tera-2, which is a human cell line that expresses IGSF4A. These findings suggest that the heterophilic binding of PVR to IGSF4A is responsible, at least in part, for the interaction between Sertoli and spermatogenic cells during mouse spermatogenesis.
Authors:
Tomohiko Wakayama; Yoshimichi Sai; Akihiko Ito; Yukio Kato; Miho Kurobo; Yoshinori Murakami; Emi Nakashima; Akira Tsuji; Yukihiko Kitamura; Shoichi Iseki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-21
Journal Detail:
Title:  Biology of reproduction     Volume:  76     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-24     Completed Date:  2007-10-02     Revised Date:  2011-08-23    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1081-90     Citation Subset:  IM    
Affiliation:
Department of Histology and Embryology, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8640, Japan. twaka@basic.m.kanazawa-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Adhesion Molecules / metabolism*
Cells, Cultured
Cloning, Molecular
Female
Immunoglobulins / genetics,  metabolism*
Male
Membrane Proteins / genetics,  metabolism*
Mice
Mice, Mutant Strains
Protein Binding
Rats
Rats, Wistar
Receptors, Virus / genetics,  metabolism*
Sertoli Cells / metabolism*
Spermatids / cytology,  metabolism*
Spermatogenesis / physiology
Tumor Suppressor Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Immunoglobulins; 0/Membrane Proteins; 0/Receptors, Virus; 0/Tumor Suppressor Proteins; 0/cell adhesion molecule 1, mouse; 0/poliovirus receptor

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