Document Detail

Heterophil function in healthy chickens and in chickens with experimentally induced staphylococcal tenosynovitis.
MedLine Citation:
PMID:  1750168     Owner:  NLM     Status:  MEDLINE    
Heterophil function was evaluated in 16 healthy chickens and in 46 chickens with experimentally induced staphylococcal tenosynovitis. In paired blood samples, heterophils from chickens with tenosynovitis had a significant increase in adherence, chemotaxis, phagocytosis, and bacterial killing of Staphylococcus aureus compared to heterophils from healthy chickens. The percent adherence of heterophils to nylon fiber columns increased significantly from a 78.4% mean +/- 6.6% standard deviation to 87.6% +/- 3.2% after induction of staphylococcal tenosynovitis. Heterophil movement following in vitro exposure to saline or endotoxin was increased in chickens with tenosynovitis; 3 +/- 1 heterophils/0.25 mm2 to 10 +/- 6 heterophils/0.25 mm2 and 136 +/- 29 heterophils/0.25 mm2 to 340 +/- 74 heterophils/0.25 mm2, respectively. Endotoxin-activated serum was chemoattractive for heterophils from all chickens. Flow cytometry was used to define the heterophil population on light scatter histograms, evaluate individual cell phagocytosis of latex beads, and quantitate the number of beads phagocytosed per heterophil. When incubated with increased numbers of beads, only heterophils from chickens with tenosynovitis phagocytosed higher numbers of beads. At heterophil to bead ratios of 1:10, the percentage of heterophils that phagocytosed beads increased from baseline values of 37.8% +/- 9.0% to post-infection values of 67.3% +/- 7.5%. Using 1:20 heterophil to bead ratios, heterophil phagocytosis increased from 38.7% +/- 9.9% to post-infection values of 79.8% +/- 7.3%. Heterophils from all chickens were able to phagocytose and kill log phase staphylococcal bacteria. After phagocytosis, the heterophils from chickens with staphylococcal tenosynovitis rapidly decreased the number of viable bacterial colony forming-units per milliliter by approximately one log.(ABSTRACT TRUNCATED AT 250 WORDS)
C B Andreasen; K S Latimer; B G Harmon; J R Glisson; J M Golden; J Brown
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Veterinary pathology     Volume:  28     ISSN:  0300-9858     ISO Abbreviation:  Vet. Pathol.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1992-01-21     Completed Date:  1992-01-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0312020     Medline TA:  Vet Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  419-27     Citation Subset:  IM    
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens.
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MeSH Terms
Cell Adhesion
Chemotaxis, Leukocyte
Flow Cytometry
Granulocytes / immunology,  physiology*
Microscopy, Electron
Poultry Diseases / blood,  immunology*
Staphylococcal Infections / blood,  immunology,  veterinary*
Staphylococcus aureus / immunology
Tenosynovitis / blood,  immunology,  veterinary*

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