| Heteronuclear 15N/1H spin echo NMR: an in vitro quantitative analysis of leucine transamination in rat skeletal muscle. | |
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MedLine Citation:
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PMID: 2543383 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Detection of the 15N nucleus in studies of the metabolism of branched-chain amino acids was carried out by recording the 1H nuclear magnetic resonance (NMR) spectrum through the effect of the 15N-1H coupling. The Selective Excitation Unit performed a 90 degrees selective proton pulse to overcome the strong water signal and baseline distorsion. In order to obtain quantitative measurement, the leucine beta protons and the valine (internal reference) beta protons coupled to 15N nucleus were simultaneously detected. This NMR method was tested on muscle homogenate incubated with [15N] leucine (approximately 3 mumoles/g). The supernatant was directly observed by NMR. The sensitivity of this indirect method was found to be far higher than direct observation of the 15N signals by 15N NMR. |
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Authors:
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D Meynial-Denis; J P Renou; M Arnal |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 160 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1989 May |
Date Detail:
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Created Date: 1989-07-10 Completed Date: 1989-07-10 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1033-9 Citation Subset: IM |
Affiliation:
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Laboratoire d'Etude du Métabolisme Azoté, URA CNRS 164, Ceyrat, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Hydrogen Kinetics Leucine / metabolism* Magnetic Resonance Spectroscopy Male Muscles / metabolism* Nitrogen Isotopes Protons Rats Rats, Inbred Strains Valine |
| Chemical | |
Reg. No./Substance:
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0/Nitrogen Isotopes; 0/Protons; 1333-74-0/Hydrogen; 61-90-5/Leucine; 7004-03-7/Valine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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