Document Detail


Heteronuclear 15N/1H spin echo NMR: an in vitro quantitative analysis of leucine transamination in rat skeletal muscle.
MedLine Citation:
PMID:  2543383     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Detection of the 15N nucleus in studies of the metabolism of branched-chain amino acids was carried out by recording the 1H nuclear magnetic resonance (NMR) spectrum through the effect of the 15N-1H coupling. The Selective Excitation Unit performed a 90 degrees selective proton pulse to overcome the strong water signal and baseline distorsion. In order to obtain quantitative measurement, the leucine beta protons and the valine (internal reference) beta protons coupled to 15N nucleus were simultaneously detected. This NMR method was tested on muscle homogenate incubated with [15N] leucine (approximately 3 mumoles/g). The supernatant was directly observed by NMR. The sensitivity of this indirect method was found to be far higher than direct observation of the 15N signals by 15N NMR.
Authors:
D Meynial-Denis; J P Renou; M Arnal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  160     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-07-10     Completed Date:  1989-07-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1033-9     Citation Subset:  IM    
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, URA CNRS 164, Ceyrat, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Hydrogen
Kinetics
Leucine / metabolism*
Magnetic Resonance Spectroscopy
Male
Muscles / metabolism*
Nitrogen Isotopes
Protons
Rats
Rats, Inbred Strains
Valine
Chemical
Reg. No./Substance:
0/Nitrogen Isotopes; 0/Protons; 1333-74-0/Hydrogen; 61-90-5/Leucine; 7004-03-7/Valine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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