Document Detail


Heteronuclear NMR analysis of unsaturated fatty acids in poly(3-hydroxyalkanoates). Study of beta-oxidation in Pseudomonas putida.
MedLine Citation:
PMID:  8416939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Poly(3-hydroxyalkanoates) (PHAs) were isolated from Pseudomonas putida KT2442 cultivated on petroselenic acid, oleic acid, and linoleic acid to study beta-oxidation of unsaturated fatty acids. Both saturated and unsaturated medium chain length 3-hydroxy fatty acids were found to be constituents of these polymers. With the aid of proton-detected multiple quantum coherence and proton-detected multiple bond coherence NMR spectra the structures of the unsaturated monomers were identified as 3-hydroxy-5-cis-tetradecanoate for PHA produced on oleic acid, and 3-hydroxy-6-cis-dodecanoate and 3-hydroxy-5-cis-8-cis-tetradecadienoate for PHA produced on linoleic acid. The identified structures, which are derived from fatty acid degradation intermediates, indicate a degradation of oleic acid via the enoyl-CoA isomerase-dependent route and a degradation of linoleic acid via the dienoyl-CoA reductase-dependent route.
Authors:
P de Waard; H van der Wal; G N Huijberts; G Eggink
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  268     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-01-28     Completed Date:  1993-01-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  315-9     Citation Subset:  IM    
Affiliation:
Agrotechnological Research Institute, Wageningen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Carbon Isotopes
Fatty Acids, Nonesterified / chemistry,  isolation & purification,  metabolism*
Gas Chromatography-Mass Spectrometry
Hydrogen
Hydroxy Acids / chemistry,  isolation & purification,  metabolism*
Linoleic Acid
Linoleic Acids / metabolism
Magnetic Resonance Spectroscopy
Molecular Conformation
Oleic Acid
Oleic Acids / metabolism
Oxidation-Reduction
Pseudomonas putida / metabolism*
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Fatty Acids, Nonesterified; 0/Hydroxy Acids; 0/Linoleic Acids; 0/Oleic Acids; 112-80-1/Oleic Acid; 1333-74-0/Hydrogen; 2197-37-7/Linoleic Acid; 4712-34-9/petroselinic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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