| Heterologously expressed β-hydroxyl fatty acids from a metagenomic library of a marine sponge. | |
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MedLine Citation:
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PMID: 23127887 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Functional screening based on the antibacterial activity of a metagenomic library of the Japanese marine sponge, Discodermia calyx, afforded three β-hydroxyl fatty acids: 3-hydroxypalmitic acid, 3-hydroxylauric acid and 3-hydroxymyristic acid, heterologously expressed in an antibacterial clone, pDC113. 3-Hydroxypalmitic acid showed moderate antibacterial activity against Bacillus cereus and Candida albicans. A sequence analysis of the insert DNA revealed 23 putative ORFs, with most sharing homology to bacterial fatty acid synthase II and lipid A biosynthesis enzymes. The other ORFs were probably transmembrane proteins involved in lipid A biosynthesis. Although lipid A was not detected under our experimental conditions, the production of β-hydroxyl fatty acids as components of lipid A were enhanced in pDC113. |
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Authors:
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Rui He; Toshiyuki Wakimoto; Yoko Egami; Hiromichi Kenmoku; Takuya Ito; Yoshinori Asakawa; Ikuro Abe |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-23 |
Journal Detail:
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Title: Bioorganic & medicinal chemistry letters Volume: - ISSN: 1464-3405 ISO Abbreviation: Bioorg. Med. Chem. Lett. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-11-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9107377 Medline TA: Bioorg Med Chem Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. |
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