Document Detail


Heterologous production of dihomo-gamma-linolenic acid in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  17873077     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To make dihomo-gamma-linolenic acid (DGLA) (20:3n-6) in Saccharomyces cerevisiae, we introduced Kluyveromyces lactis Delta12 fatty acid desaturase, rat Delta6 fatty acid desaturase, and rat elongase genes. Because Fad2p is able to convert the endogenous oleic acid to linoleic acid, this allowed DGLA biosynthesis without the need to supply exogenous fatty acids on the media. Medium composition, cultivation temperature, and incubation time were examined to improve the yield of DGLA. Fatty acid content was increased by changing the medium from a standard synthetic dropout medium to a nitrogen-limited minimal medium (NSD). Production of DGLA was higher in the cells grown at 15 degrees C than in those grown at 20 degrees C, and no DGLA production was observed in the cells grown at 30 degrees C. In NSD at 15 degrees C, fatty acid content increased up until day 7 and decreased after day 10. When the cells were grown in NSD for 7 days at 15 degrees C, the yield of DGLA reached 2.19 microg/mg of cells (dry weight) and the composition of DGLA to total fatty acids was 2.74%. To our knowledge, this is the first report describing the production of polyunsaturated fatty acids in S. cerevisiae without supplying the exogenous fatty acids.
Authors:
Hisashi Yazawa; Hitoshi Iwahashi; Yasushi Kamisaka; Kazuyoshi Kimura; Tsunehiro Aki; Kazuhisa Ono; Hiroshi Uemura
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Publication Detail:
Type:  Journal Article     Date:  2007-09-14
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  73     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-30     Completed Date:  2008-03-25     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6965-71     Citation Subset:  IM    
Affiliation:
National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
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MeSH Terms
Descriptor/Qualifier:
8,11,14-Eicosatrienoic Acid / metabolism*
Animals
Culture Media / chemistry
Fatty Acid Desaturases / genetics,  metabolism*
Fatty Acids, Unsaturated / biosynthesis*,  metabolism
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Rats
Recombinant Proteins / biosynthesis,  genetics
Recombination, Genetic
Saccharomyces cerevisiae / enzymology,  metabolism*
Temperature
Chemical
Reg. No./Substance:
0/Culture Media; 0/Fatty Acids, Unsaturated; 0/Recombinant Proteins; 7324-41-6/8,11,14-Eicosatrienoic Acid; EC 1.14.19.-/Fatty Acid Desaturases
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