| Heterologous production of dihomo-gamma-linolenic acid in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 17873077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To make dihomo-gamma-linolenic acid (DGLA) (20:3n-6) in Saccharomyces cerevisiae, we introduced Kluyveromyces lactis Delta12 fatty acid desaturase, rat Delta6 fatty acid desaturase, and rat elongase genes. Because Fad2p is able to convert the endogenous oleic acid to linoleic acid, this allowed DGLA biosynthesis without the need to supply exogenous fatty acids on the media. Medium composition, cultivation temperature, and incubation time were examined to improve the yield of DGLA. Fatty acid content was increased by changing the medium from a standard synthetic dropout medium to a nitrogen-limited minimal medium (NSD). Production of DGLA was higher in the cells grown at 15 degrees C than in those grown at 20 degrees C, and no DGLA production was observed in the cells grown at 30 degrees C. In NSD at 15 degrees C, fatty acid content increased up until day 7 and decreased after day 10. When the cells were grown in NSD for 7 days at 15 degrees C, the yield of DGLA reached 2.19 microg/mg of cells (dry weight) and the composition of DGLA to total fatty acids was 2.74%. To our knowledge, this is the first report describing the production of polyunsaturated fatty acids in S. cerevisiae without supplying the exogenous fatty acids. |
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Authors:
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Hisashi Yazawa; Hitoshi Iwahashi; Yasushi Kamisaka; Kazuyoshi Kimura; Tsunehiro Aki; Kazuhisa Ono; Hiroshi Uemura |
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Publication Detail:
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Type: Journal Article Date: 2007-09-14 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 73 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-10-30 Completed Date: 2008-03-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 6965-71 Citation Subset: IM |
Affiliation:
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National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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8,11,14-Eicosatrienoic Acid
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metabolism* Animals Culture Media / chemistry Fatty Acid Desaturases / genetics, metabolism* Fatty Acids, Unsaturated / biosynthesis*, metabolism Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Rats Recombinant Proteins / biosynthesis, genetics Recombination, Genetic Saccharomyces cerevisiae / enzymology, metabolism* Temperature |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids, Unsaturated; 0/Recombinant Proteins; 7324-41-6/8,11,14-Eicosatrienoic Acid; EC 1.14.19.-/Fatty Acid Desaturases |
| Comments/Corrections | |
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