Document Detail

Heterologous expression of a bacterial haemoglobin improves the growth properties of recombinant Escherichia coli.
MedLine Citation:
PMID:  3277067     Owner:  NLM     Status:  MEDLINE    
Rational design of novel as well as improved cellular biocatalysts by genetic manipulation of cellular metabolism has recently attracted considerable interest. A wide range of bacteria have been genetically modified by integrating new enzymatic functions into their metabolic network. A central problem in the aerobic growth of any cell culture is the maintenance of dissolved oxygen (DO) concentrations above growth-limiting levels especially in high cell-density fermentations which are usually of a fed-batch type. The optimal rate of nutrient addition (and consequently the productivity) is ultimately limited by the rate at which cells can aerobically catabolize the carbon source without generating growth-inhibitory metabolites such as lactate and acetate. All approaches thus far have concentrated on improving the oxygen mass transfer rates by manipulating various environmental parameters. We have isolated the gene for a haemoglobin-like molecule, expressed by the aerobic bacterium Vitreoscilla in poorly-oxygenated environments, and expressed it in Escherichia coli. The recombinant cells contain enhanced haem as well as active haemoglobin, and they grow faster and to considerably greater cell densities than comparable plasmid-containing cells which do not express haemoglobin. This haemoglobin increases the rate of oxygen use, especially when dissolved oxygen is less than 5% of air saturation.
C Khosla; J E Bailey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Nature     Volume:  331     ISSN:  0028-0836     ISO Abbreviation:  Nature     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-03-23     Completed Date:  1988-03-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  633-5     Citation Subset:  IM    
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.
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MeSH Terms
Bacterial Proteins / physiology*
Escherichia coli / growth & development*,  metabolism
Hemoglobins / physiology*
Oxygen / metabolism
Recombinant Proteins / physiology*
Reg. No./Substance:
0/Bacterial Proteins; 0/Hemoglobins; 0/Recombinant Proteins; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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