Document Detail

Heterogeneously Expressed fezf2 Patterns Gradient Notch Activity in Balancing the Quiescence, Proliferation, and Differentiation of Adult Neural Stem Cells.
MedLine Citation:
PMID:  25319688     Owner:  NLM     Status:  In-Data-Review    
Balancing quiescence, self-renewal, and differentiation in adult stem cells is critical for tissue homeostasis. The underlying mechanisms, however, remain incompletely understood. Here we identify Fezf2 as a novel regulator of fate balance in adult zebrafish dorsal telencephalic neural stem cells (NSCs). Transgenic reporters show intermingled fezf2-GFP(hi) quiescent and fezf2-GFP(lo) proliferative NSCs. Constitutive or conditional impairment of fezf2 activity demonstrates its requirement for maintaining quiescence. Analyses of genetic chimeras reveal a dose-dependent role of fezf2 in NSC activation, suggesting that the difference in fezf2 levels directionally biases fate. Single NSC profiling coupled with genetic analysis further uncovers a fezf2-dependent gradient Notch activity that is high in quiescent and low in proliferative NSCs. Finally, fezf2-GFP(hi) quiescent and fezf2-GFP(lo) proliferative NSCs are observed in postnatal mouse hippocampus, suggesting possible evolutionary conservation. Our results support a model in which fezf2 heterogeneity patterns gradient Notch activity among neighbors that is critical to balance NSC fate.
Michael A Berberoglu; Zhiqiang Dong; Guangnan Li; Jiashun Zheng; Luz Del Carmen G Trejo Martinez; Jisong Peng; Mahendra Wagle; Brian Reichholf; Claudia Petritsch; Hao Li; Samuel J Pleasure; Su Guo
Related Documents :
25404388 - Feline foamy virus adversely affects feline mesenchymal stem cell culture and expansion...
25482558 - Sox2 acts in a dose-dependent fashion to regulate proliferation of cortical progenitors.
1983968 - Conserved t cell receptor v gene usage by uveitogenic t cells.
25273538 - The autism spectrum disorders stem cell resource at children's hospital of orange count...
20629528 - Monoclonal b cell lymphocytosis.
864758 - Immune response to l2 guinea pig leukemia-specific antigens.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  34     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13911-23     Citation Subset:  IM    
Copyright Information:
Copyright © 2014 the authors 0270-6474/14/3413911-13$15.00/0.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Role of glutamatergic projections from ventral tegmental area to lateral habenula in aversive condit...
Next Document:  Selective activation of ipsilateral motor pathways in intact humans.