Document Detail

Heterogeneous expression of blood group A-determinant in a human gastric cancer cell line derived from a blood group A individual.
MedLine Citation:
PMID:  1373646     Owner:  NLM     Status:  MEDLINE    
The human gastric cancer cell line MKN 45 was derived from the tumour of a blood group A individual, and was known to express large quantities of blood group A-antigen. Using immunofluorescence we found the MKN 45 cells, donated from the Japanese Cancer Research Resources Bank, consisted of A-antigen positive cells (18%) and A-antigen negative cells (82%). After limiting dilution, wild type and mutant cells were cloned with regard to the expression of a cell surface A-antigen. ELISA was used to detect A-antigen in the cell extract of the wild type cells, but none was evident in those of the mutant cells. However, blood group A-gene-specified transferase activity of the mutant cells was comparable to that of the wild type cells.
Y Kominato; T Fujikura; H Takizawa; K Hayashi; M Fujimaki; K Kishi; T Suzuki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics     Volume:  19     ISSN:  0960-7420     ISO Abbreviation:  Eur. J. Immunogenet.     Publication Date:    1992 Feb-Apr
Date Detail:
Created Date:  1992-05-27     Completed Date:  1992-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9106962     Medline TA:  Eur J Immunogenet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1-9     Citation Subset:  IM    
Department of Legal Medicine, Toyama Medical & Pharmaceutical University, Japan.
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MeSH Terms
ABO Blood-Group System / biosynthesis*,  genetics
Epitopes / biosynthesis,  genetics
Fluorescent Antibody Technique
Gene Expression
Glycosyltransferases / genetics
Polymorphism, Genetic
Stomach Neoplasms / genetics,  immunology*
Tumor Cells, Cultured / enzymology,  immunology
Reg. No./Substance:
0/ABO Blood-Group System; 0/Epitopes; EC 2.4.-/Glycosyltransferases

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