| Heterogeneous set of cell wall teichoic acids in strains of Bacillus subtilis VKM B-760 and VKM B-764. | |
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MedLine Citation:
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PMID: 19961419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell walls of Bacillus subtilis VKM B-760 and VKM B-764 are characterized by heterogeneous composition of teichoic acids. Polymer I with structure -6)-beta-D-Galp-(1-->1)-sn-Gro-(3-P-, polymer II with structure -6)-alpha-D-Glcp-(1-->1)-sn-Gro-(3-P-, and a small amount of unsubstituted 1,3-poly(glycerol phosphate) were detected in strain VKM B-760. Strain VKM B-764 contains an analogous set of teichoic acids, but a characteristic feature of polymer II is the presence of disubstituted glycerol residue with alpha-glucopyranose localization in the integral chain at C-1 hydroxyl and beta-glucopyranose as a side branch at C-2 hydroxyl (polymer III): -6)-alpha-D-Glcp-(1-->1)-[beta-D-Glcp-(1-->2)]-sn-Gro-(3-P-. The structures of polymer I in bacilli and polymer III in Gram-positive bacteria are described for the first time. Teichoic acids were studied by chemical methods and on the basis of combined analysis of one-dimensional 1H-, 13C-, and (31)P-NMR spectra, homonuclear two-dimensional (1)H/(1)H COSY, TOCSY, and ROESY, and heteronuclear two-dimensional (1)H/(13)C gHSQC- and HMQC-TOCSY experiments. Simultaneous presence of several different structure teichoic acids in the bacillus cell walls as well as chemotaxonomical perspectives of the application of these polymers as species-specific markers for members of the Bacillus genus is discussed. |
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Authors:
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N V Potekhina; G M Streshinskaya; Yu I Kozlova; E B Kudryashova; S N Senchenkova; A S Shashkov; L N Anan'ina |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry. Biokhimii?a Volume: 74 ISSN: 1608-3040 ISO Abbreviation: Biochemistry Mosc. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-07 Completed Date: 2010-03-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376536 Medline TA: Biochemistry (Mosc) Country: United States |
Other Details:
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Languages: eng Pagination: 1368-74 Citation Subset: IM |
Affiliation:
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Faculty of Biology, Lomonosov Moscow State University, Moscow, 119899, Russia. potekchina@hotbox.ru |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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chemistry* Carbohydrate Sequence Cell Wall / chemistry* Magnetic Resonance Spectroscopy Teichoic Acids / analysis, chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Teichoic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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