Document Detail


Heterogeneous phenotype of human melanoma cells with in vitro and in vivo features of tumor-initiating cells.
MedLine Citation:
PMID:  20376064     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Melanospheres, the melanoma cells that grow as nonadherent colonies and that show in vitro self-renewing capacity and multipotency, were selected from melanoma specimens or from melanoma cell lines. Melanospheres were highly tumorigenic, and intradermal injections in severe combined immunodeficient (SCID) mice of as few as 100 cells generated tumors that maintained tumorigenic potential into subsequent recipients. Primary and serially transplanted xenografts recapitulated the phenotypic features of the original melanoma of the patient. Melanoma cells cultured in the presence of fetal calf serum (FCS) were also tumorigenic in SCID mice, although with lower efficiency; these xenografts showed a homogeneous phenotype for the expression of melanoma-associated markers, Melan-A/Mart-1, HMB45, and MITF, and contained cells with features of fully differentiated cells. Melanospheres were heterogeneous for the expression of stem cell markers and showed a significantly enhanced expression of the Nanog and Oct3/4 transcription factors when compared with adherent melanoma cells. No direct and unique correlation between any of the examined stem cell markers and in vivo tumorigenicity was found. Taken together, our data provide further evidence on the heterogeneous nature of human melanomas and show that melanospheres and their corresponding tumors, which are generated in vivo in immunocompromised mice, represent a model to investigate melanoma biology.
Authors:
Michela Perego; Monica Tortoreto; Gabrina Tragni; Luigi Mariani; Paola Deho; Antonino Carbone; Mario Santinami; Roberto Patuzzo; Pamela Della Mina; Antonello Villa; Graziella Pratesi; Giacomo Cossa; Paola Perego; Maria G Daidone; Malcolm R Alison; Giorgio Parmiani; Licia Rivoltini; Chiara Castelli
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-08
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  130     ISSN:  1523-1747     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-15     Completed Date:  2010-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1877-86     Citation Subset:  IM    
Affiliation:
Department of Experimental Oncology, Fondazione IRCSS, Istituto Nazionale dei Tumori, Milan, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD146 / metabolism
Blood Proteins / pharmacology
Cell Differentiation / physiology
Cell Line, Tumor
Disease Models, Animal*
Homeodomain Proteins / metabolism
Humans
Immunophenotyping
Lymphatic Metastasis
Melanoma / physiopathology,  secondary*
Mice
Mice, SCID*
Neoplasm Transplantation / methods*
Neoplastic Stem Cells / pathology
Octamer Transcription Factor-3 / metabolism
Skin Neoplasms / pathology*,  physiopathology
Spheroids, Cellular
Transplantation, Heterologous
Tumor Markers, Biological / metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD146; 0/Blood Proteins; 0/Homeodomain Proteins; 0/MCAM protein, human; 0/Nanog protein, mouse; 0/Octamer Transcription Factor-3; 0/Pou5f1 protein, mouse; 0/Tumor Markers, Biological
Comments/Corrections
Comment In:
J Invest Dermatol. 2010 Jul;130(7):1769-71   [PMID:  20548315 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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