Document Detail


Heterogeneity of pregnancy-specific beta 1-glycoprotein following purification from maternal serum and partial characterization of a high-molecular-weight component.
MedLine Citation:
PMID:  6974239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pregnancy-Specific beta 1-Glycoprotein (PSB1G) has been purified from maternal serum by physico-chemical and immunochemical methods. Marked instability of the protein was noted after partial purification which correlated with hydroxylapatite chromatography of PSB1G. Three molecular heterogeneous forms were demonstrated by gel filtration on Sephacryl S-200 which were designated as PSB1G-I, II and III. Each of the latter purified materials displayed altered immunoelectrophoretic mobility, i.e., altered from beta 1 to gamma mobility. In immunodiffusion experiments PSB1G-I reacted in complete immunological identity with 'native' serum PSB1G whereas PSB1G-II and III reacted in partial identity with each of the latter, suggesting that they are dissociation and/or degradation products of the parent PSB1G. The apparent molecular weights of PSB1G-I, II and III as determined by gel filtration were 280 000, 69 000 and 23 000, respectively, PSB1G-I was selected for structural analysis on the basis of its immunological identity with 'native' antigen. SDS-PAGE studies of PSB1G-I with and without reducing agent strongly suggest that PSB1G-I and 'native' serum antigen are composed of two dissimilar subunits of equal molecular size which are bonded together by non-covalent linkage. One of the subunits appears to represent a single polypeptide chain, whereas the other subunit appears to be composed of two polypeptide chains of unequal size that are linked together by covalent bonds. Investigations are continuing towards confirmation of the proposed structural model of PSB1G-I by an alternate methodology of protein structure analysis.
Authors:
B W Griffiths; A Godard
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of reproductive immunology     Volume:  3     ISSN:  0165-0378     ISO Abbreviation:  J. Reprod. Immunol.     Publication Date:  1981 Jun 
Date Detail:
Created Date:  1981-11-22     Completed Date:  1981-11-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8001906     Medline TA:  J Reprod Immunol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  117-29     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blood Protein Electrophoresis
Chemical Phenomena
Chemistry
Chromatography, Affinity
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Female
Humans
Immunoelectrophoresis
Molecular Weight
Pregnancy
Pregnancy Proteins / blood*
Pregnancy-Specific beta 1-Glycoproteins / analysis,  blood*
Chemical
Reg. No./Substance:
0/Pregnancy Proteins; 0/Pregnancy-Specific beta 1-Glycoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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