Document Detail


Heterogeneity of phospholipid synthesis in rat liver endoplasmic reticulum during proliferation of smooth membranes.
MedLine Citation:
PMID:  185227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During proliferation of smooth endoplasmic reticulum (SER) induced by phenobarbital the specific activity of acyltransferases of the smooth microsomes increases, there is a transient rise in the phospholipid/protein ratio of these membranes, and an increased incorporation of [14C]glycerol into smooth-membrane phospholipid. Microsomes separated into subfractions on 2 gradients exhibited a heterogeneous distribution of these characteristics, indicating a non-uniform distribution of the site of phospholipid synthesis in the ER under these conditions. Cytochemical localization of acyltransferases on whole liver and smooth and rough microsomes confirmed this heterogeneity, and indicated that the distribution of this activity was not restricted to any morphologically distinct site in the ER of the intact cell. After 4 days of phenobarbital treatment the increased membrane is restricted to lighter subfractions and is similar in distribution to that of increased acyltransferase activity. These results indicate that the synthesis of membrane phospholipid and the growth of the SER in response to phenobarbital is not uniform but occurs at randomly dispersed sites in the SER while proteins may be added preferentially at these sites resulting in a final uniform distribution.
Authors:
J A Higgins
Related Documents :
19458387 - Depletion of phosphatidylcholine affects endoplasmic reticulum morphology and protein t...
6563037 - Density of newly synthesized plasma membrane proteins in intracellular membranes. i. st...
3448617 - Three dimensional visualization of the golgi apparatus and endoplasmic reticulum in the...
12475947 - Specific heterodimer formation is a prerequisite for uroplakins to exit from the endopl...
6887157 - The development of transport ability by embryonic follicle-associated epithelium.
9479007 - Mutation of a conserved proline disrupts the retinal-binding pocket of the x-linked con...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cell science     Volume:  22     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1976 Oct 
Date Detail:
Created Date:  1976-12-30     Completed Date:  1976-12-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  173-97     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acyltransferases / analysis,  metabolism
Animals
Endoplasmic Reticulum / metabolism*
Glucose-6-Phosphatase / analysis
Glycerol / metabolism
Histocytochemistry
Liver / metabolism*,  ultrastructure
Microsomes / enzymology,  ultrastructure
Microsomes, Liver / metabolism
Nucleotidases / analysis
Phenobarbital / pharmacology
Phospholipids / biosynthesis*
Proteins / metabolism
Rats
Chemical
Reg. No./Substance:
0/Phospholipids; 0/Proteins; 50-06-6/Phenobarbital; 56-81-5/Glycerol; EC 2.3.-/Acyltransferases; EC 3.1.3.-/Nucleotidases; EC 3.1.3.9/Glucose-6-Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Ascorbate-independent proline hydroxylation resulting from viral transformation of Balb 3T3 cells an...
Next Document:  A theoretical study of the effects of cyclic AMP phosphodiesterases during aggregation in Dictyostel...