Document Detail


Heterogeneity of nascent high density lipoproteins secreted by the hepatoma-derived cell line, Hep G2.
MedLine Citation:
PMID:  2846733     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nondenaturing gradient gel analysis of high density lipoproteins (HDL, d 1.063-1.235 g/ml) isolated from Hep G2 24-hr, serum-free conditioned media shows four distinct, reproducible particle subclasses I, II, III, and IV with apparent Stokes' diameters of 13.3, 12.0, 9.5, and 7.4 nm, respectively. Fractions enriched in lipoproteins from each of these subclasses were isolated by either density gradient ultracentrifugation or gel filtration chromatography and characterized. Size and morphology of the isolated subclasses agreed well regardless of isolation procedure. Electron microscopy revealed subclasses I, II, and III to be disc-shaped, and subclass IV to be spherical. The discoidal subclasses were poor in cholesteryl ester and rich in phospholipid and unesterified cholesterol. The larger-sized subclass I particles were enriched in apolipoprotein (apo) E while subclasses II and III had decreasing amounts of apoE and increasing amounts of apoA-I and A-II. The spherical subclass IV particles contained a higher percentage of protein and had a higher ratio of cholesteryl ester to unesterified cholesterol than that found in the other subclasses. Subclass IV contained predominantly apoA-I. The subclasses isolated from Hep G2 HDL appear to share many similarities with those isolated from patients with lecithin:cholesterol acyltransferase deficiency and are therefore potentially useful in examining the transformation of nascent HDL particles to mature circulating plasma forms.
Authors:
M R McCall; T M Forte; V G Shore
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  29     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1988-12-20     Completed Date:  1988-12-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1127-37     Citation Subset:  IM    
Affiliation:
Donner Laboratory, University of California, Berkeley 94720.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Carcinoma, Hepatocellular / analysis*,  ultrastructure
Centrifugation, Density Gradient
Chromatography, Gel
Humans
Lipoproteins, HDL / analysis*
Liver Neoplasms
Microscopy, Electron
Tumor Cells, Cultured / analysis,  ultrastructure
Ultracentrifugation
Grant Support
ID/Acronym/Agency:
HL-07279/HL/NHLBI NIH HHS; HL-18574/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Lipoproteins, HDL

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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