| Heterogeneity of mitochondrial potential as a marker for isolation of pure cardiomyoblast population. | |
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MedLine Citation:
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PMID: 19489330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Typical signs of cardiomyoblasts were determined: high mitochondrial membrane potential and high number of mitochondria in these cells compared to fibroblasts. These signs can be used for identification of these cells. Energy-dependent accumulation of highly specific mitochondrial fluorescent probes applied for visual detection of energized mitochondria allows clear-cut separation of the mixed population: cardiomyocyte population is characterized by higher transmembrane potential than concomitant cells. Using flow cytometry and cell sorting we obtained a population enriched with cardiomyocytes and cardiomyoblasts. Taking into account the fact that the dye has no toxic effect on cells and is rapidly eliminated, the risk of cell damage and death during isolation is considerably reduced compared to traditional methods. These results open possibilities for the development of a new specific method for isolation of cardiomyocyte culture from fetal and embryonic sources for their further use in clinical practice. |
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Authors:
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T G Khryapenkova; E Y Plotnikov; M V Korotetskaya; G T Sukhikh; D B Zorov |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bulletin of experimental biology and medicine Volume: 146 ISSN: 0007-4888 ISO Abbreviation: Bull. Exp. Biol. Med. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2009-06-03 Completed Date: 2009-06-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372557 Medline TA: Bull Exp Biol Med Country: United States |
Other Details:
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Languages: eng Pagination: 506-11 Citation Subset: IM |
Affiliation:
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Faculty of Bioengineering and Bioinformatics, A. N. Belozerskii Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University, Moscow, Russia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers Cells, Cultured Flow Cytometry Membrane Potential, Mitochondrial* Myoblasts, Cardiac / cytology*, metabolism Myocytes, Cardiac / cytology, metabolism Rats |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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