Document Detail


Heterogeneity of the inhibitory effects of IL-4 in two novel B lineage acute lymphoblastic leukemia cell lines.
MedLine Citation:
PMID:  9444937     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study describes two novel cell lines, DUNATIS and SILVANUS, established from B lineage acute lymphoblastic leukemia patients. Respectively, DUNATIS and SILVANUS display an early pre-B cell and a pre-B cell phenotype. Spontaneous DNA replication of both cell lines was strongly inhibited by IL-4. This effect was directly mediated by IL-4 and exerted through the CD124 IL-4 receptor chain. Notably, IL-4 was associated with rapid cell death and reduction of cellularity in DUNATIS, whereas these parameters were considerably less pronounced and only observed after longer-term exposure of the SILVANUS cells to IL-4. In addition to these differences, although both cell lines expressed FES oncoprotein, a 100 kDa protein associated with FES was strikingly found to be tyrosine-phosphorylated in response to IL-4 exclusively in DUNATIS cells. These data demonstrate that IL-4 displays heterogenous effects on leukemic B cell precursors responsive to inhibition of DNA synthesis via IL-4 mediated engagement of the CD124 receptor chain. The present findings may be of use for appreciation of the effects of IL-4 in B lineage ALL, and the novel cell lines could represent a model for further identification of target molecules in IL-4 signalling.
Authors:
N Renard; N Harada; E Callet-Bauchu; A Miyajima; V Duvert; J Banchereau; S Saeland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia research     Volume:  21     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:    1997 Nov-Dec
Date Detail:
Created Date:  1998-02-10     Completed Date:  1998-02-10     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1037-46     Citation Subset:  IM    
Affiliation:
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Burkitt Lymphoma / drug therapy*,  metabolism,  pathology*
Cell Division / drug effects
Cell Survival / drug effects
DNA Replication / drug effects
DNA, Neoplasm / biosynthesis,  drug effects
Female
Humans
Interleukin-4 / pharmacology*
Male
Neoplasm Proteins / metabolism
Phenotype
Phosphorylation / drug effects
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / drug therapy*,  metabolism,  pathology*
Protein-Tyrosine Kinases*
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins c-fes
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Neoplasm Proteins; 0/Proto-Oncogene Proteins; 207137-56-2/Interleukin-4; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.1/Proto-Oncogene Proteins c-fes; EC 2.7.10.2/FES protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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