Document Detail


Heterogeneity in copper and glycan content of ceruloplasmin in human serum differs in health and disease.
MedLine Citation:
PMID:  3234365     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Crossed immunoelectrophoresis of human serum revealed two heterogeneity types of ceruloplasmin with different electrophoretic migration. The two types both consisted of peptides with Mr 150,000, 100,000 and 45,000, which were interpreted as native ceruloplasmin and two hydrolytic fragments. The two types were different in copper content, and one type could reversibly be changed into the other. The glycan microheterogeneity of ceruloplasmin was analyzed by crossed affinoimmunoelectrophoresis with free Lens culinaris agglutinin (LCA) and wheat germ agglutinin (WGA). A third of the ceruloplasmin molecules, both high and low copper type, bound to LCA and two thirds to WGA. The heterogeneity and the microheterogeneity of ceruloplasmin in two groups of patient sera were compared to sera from healthy individuals. The ceruloplasmin type with respect to copper content was a much better factor than either glycan microheterogeneity or total serum concentration in discriminating between the three groups.
Authors:
J E Hansen; P M Heegaard; S P Jensen; B Nørgaard-Pedersen; T C Bøg-Hansen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  9     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1989-04-27     Completed Date:  1989-04-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY, EAST    
Other Details:
Languages:  eng     Pagination:  273-8     Citation Subset:  IM    
Affiliation:
Protein Laboratory, University of Copenhagen, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Ceruloplasmin / analysis*
Chromatography, Affinity
Copper / blood*
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Humans
Immunoblotting
Immunoelectrophoresis, Two-Dimensional
Infant, Newborn
Lectins / isolation & purification
Molecular Weight
Plant Lectins*
Polysaccharides / blood*
Chemical
Reg. No./Substance:
0/Lectins; 0/Plant Lectins; 0/Polysaccharides; 0/lentil lectin; 7440-50-8/Copper; EC 1.16.3.1/Ceruloplasmin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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