Document Detail

Heterogeneity in c-jun gene expression in normal and malignant cells exposed to either ionizing radiation or hydrogen peroxide.
MedLine Citation:
PMID:  7724734     Owner:  NLM     Status:  MEDLINE    
We investigated the role of reactive oxygen intermediates and protein kinase C in the induction of expression of the c-jun gene in human ML-2 leukemic cells and normal human DET-551 fibroblasts by comparing the effects of exposure to either ionizing radiation or H2O2 in the presence or absence of appropriate inhibitors. In these cell types, the radiation- and H2O2-mediated increase in c-jun mRNA levels could be prevented by pretreatment of the cells with N-acetylcysteine, an antioxidant, or H7, an inhibitor of protein kinase C and protein kinase A, but not by HA1004, a specific inhibitor of protein kinase A and G. These results suggest a role for protein kinase C and reactive oxygen intermediates in the induction of c-jun gene expression in both normal and tumor cells. We also investigated potential differences in c-jun gene expression induced by radiation or H2O2 in normal and tumor cells by examining steady-state c-jun mRNA levels in a number of human fibroblast, leukemia, melanoma, sarcoma and carcinoma cell types. We observed heterogeneity in the steady-state level of c-jun mRNA in both the untreated normal and tumor cells and in such cells exposed to ionizing radiation or to H2O2. Exposure to radiation produced a varied response which ranged from little or no induction to an increase in the steady-state level of the c-jun mRNA of more than two orders of magnitude. Exposure to H2O2 gave a pattern similar to that of ionizing radiation. The basis for the differential induction in response to these agents may be attributable to either cell lineage or genetic heterogeneity or a combination of these two parameters.
F R Collart; M Horio; E Huberman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Radiation research     Volume:  142     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-05-25     Completed Date:  1995-05-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  188-96     Citation Subset:  IM; S    
Center For Mechanistic Biology and Biotechnology, Argonne National Laboratory, Illinois 60439, USA.
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MeSH Terms
Base Sequence
Dose-Response Relationship, Radiation
Gene Expression Regulation / drug effects,  radiation effects*
Genes, jun*
Hydrogen Peroxide / pharmacology*
Molecular Sequence Data
Protein Kinase C / physiology
RNA, Messenger / analysis
Reactive Oxygen Species
Tumor Cells, Cultured
Reg. No./Substance:
0/RNA, Messenger; 0/Reactive Oxygen Species; 7722-84-1/Hydrogen Peroxide; EC Kinase C

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