Document Detail

Heterogeneity in the activity of Mexican Helicobacter pylori strains in gastric epithelial cells and its association with diversity in the cagA gene.
MedLine Citation:
PMID:  17438024     Owner:  NLM     Status:  MEDLINE    
Helicobacter pylori CagA is translocated into gastric epithelial cells by a type IV secretion system and interacts with the Src homology 2 phosphatase, altering cell morphology. Multiple EPIYA motifs in CagA are associated with increased activity in cells and with gastric cancer. The aim of this work was to study the heterogeneity in activity in cells of multiple H. pylori single colonies isolated from a single patient and its association with polymorphism in cagA. The presence of cagA, cagE, cagT, and cag10 was studied with 318 H. pylori isolates from the antra and corpora of 18 patients. AGS gastric epithelial cells were infected with 75 isolates, and interleukin-8 (IL-8) secretion, cytoskeletal changes, CagA translocation, and tyrosine phosphorylation were measured. The cagA 3'-variable region was sequenced for 30 isolates to determine the number and types of EPIYA motifs. Isolates from an individual stomach were usually genetically related and had quantitatively similar phenotypic effects on cells (IL-8 induction and cytoskeletal changes). However, strains from different patients with similar CagA EPIYA motif patterns varied widely in these phenotypes. Among isolates with an EPIYA-ABC pattern, the phenotype was variable: IL-8 induction ranged from 200 to 1,200 pg/ml, and morphological changes occurred in 20 to 70% of cells. In several cases, cagA sequence diversity appeared to explain the lack of CagA activity, as isolates with an EPIYA-ACC pattern or a modified B motif had reduced cell activity. cag pathogenicity island-positive H. pylori isolates displayed a high level of heterogeneity in the capacity to induce IL-8 secretion and morphological changes; an absent or modified B motif was associated with low activity.
Adriana Reyes-Leon; John C Atherton; Richard H Argent; J L Puente; J Torres
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-16
Journal Detail:
Title:  Infection and immunity     Volume:  75     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-20     Completed Date:  2007-08-07     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3445-54     Citation Subset:  IM    
Unidad de Investigación Medica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social (IMSS), Av. Cuauhtemoc 330, Mexico, DF CP 06725, Mexico.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EF552407;  EF552408;  EF552409;  EF552410;  EF552411;  EF552412;  EF552413;  EF552414;  EF552415;  EF552416;  EF552417;  EF552418;  EF552419;  EF552420;  EF552421;  EF552422;  EF552423;  EF552424
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MeSH Terms
Aged, 80 and over
Amino Acid Sequence
Antigens, Bacterial / chemistry,  genetics*,  metabolism
Bacterial Proteins / chemistry,  genetics*,  metabolism
Cell Line
Epithelial Cells / immunology,  microbiology*
Genetic Variation*
Helicobacter Infections / microbiology
Helicobacter pylori / classification*,  genetics,  isolation & purification,  pathogenicity*
Interleukin-8 / biosynthesis
Middle Aged
Molecular Sequence Data
Sequence Analysis, DNA
Stomach / cytology*,  immunology
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Interleukin-8; 0/cagA protein, Helicobacter pylori

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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