Document Detail

Heterogeneity of human peripheral blood eosinophil-type colonies: evidence for a common basophil-eosinophil progenitor.
MedLine Citation:
PMID:  2410064     Owner:  NLM     Status:  MEDLINE    
We have recently shown that a proportion of previously designated human eosinophil "(Eo)-type" colonies in methylcellulose contain basophils and histamine (Denburg et al Blood 61:775, 1983). In the present studies, individual Eo-type colonies have been analyzed by cell morphology as well as by biochemical assays for histamine, Charcot-Leyden crystal protein (CLC), and eosinophil granule major basic protein (MBP). Clonal origin of single Eo-type colonies was confirmed by G6PD isoenzyme analysis. Morphological observations of such colonies revealed the existence of two distinct colony types: (1) Eo type containing 100% basophils and (2) Eo type containing mixtures of basophils and eosinophils, including cells with mixed basophil-eosinophil granulation. Histamine was not detected in pure, mature peripheral blood eosinophils. Immunofluorescent studies demonstrated bright staining for CLC and MBP in 95% +/- 3% of cells in Eo-type colonies but only in 5% +/- 4% of cells in GM-type colonies. Radioimmunoassay for MBP was positive in 5/9 Eo-type and 0/10 neutrophil-macrophage ("GM-type") colonies, with a mean level (nanogram/colony) of 11.6 +/- 4.2 per Eo-type colony; four of the latter colonies were doubly positive for both histamine and MBP. These and previous findings point out the morphological and biochemical heterogeneity of peripheral blood Eo-type colonies and provide direct evidence for the existence of a common, circulating basophil-eosinophil progenitor.
J A Denburg; S Telizyn; H Messner; B Lim; N Jamal; S J Ackerman; G J Gleich; J Bienenstock
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Blood     Volume:  66     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1985 Aug 
Date Detail:
Created Date:  1985-09-11     Completed Date:  1985-09-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  312-8     Citation Subset:  AIM; IM    
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MeSH Terms
Basophils / cytology*
Blood Cell Count
Blood Proteins / analysis
Cells, Cultured
Eosinophil Granule Proteins
Eosinophilia / blood
Eosinophils / cytology*
Fluorescent Antibody Technique
Glucosephosphate Dehydrogenase / analysis
Glycoproteins / analysis
Histamine / analysis
Hypersensitivity, Immediate / blood
Staining and Labeling
Stem Cells / cytology*
Reg. No./Substance:
0/Blood Proteins; 0/Eosinophil Granule Proteins; 0/Glycoproteins; 0/lysolecithin acylhydrolase; 51-45-6/Histamine; 9004-67-5/Methylcellulose; EC Dehydrogenase; EC 3.1.-/Ribonucleases; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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