Document Detail


Heterogeneity of genome sizes within the genus Spiroplasma.
MedLine Citation:
PMID:  7857799     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Organisms belonging to the genus Spiroplasma are currently classified into 23 groups, 17 of which have been assigned species epithets. We determined the genome sizes of representatives of 20 groups by using pulsed-field gel electrophoresis. Each genome size was deduced from the mobility of linear nonrestricted DNA, as well as from the sum of the sizes of restriction fragments obtained after digestion with NotI, a restriction endonuclease with a limited number of restriction sites in spiroplasma DNA. The values which we obtained indicated that the genome sizes of members of the genus Spiroplasma range from 940 to 2,220 kbp.
Authors:
P Carle; F Laigret; J G Tully; J M Bové
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of systematic bacteriology     Volume:  45     ISSN:  0020-7713     ISO Abbreviation:  Int. J. Syst. Bacteriol.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-03-23     Completed Date:  1995-03-23     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0042143     Medline TA:  Int J Syst Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  178-81     Citation Subset:  IM    
Affiliation:
Laboratoire de Biologie Cellulaire et Moléculaire, Institut National de la Recherche Agronomique, Villenave d'Ornon.
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MeSH Terms
Descriptor/Qualifier:
Genome, Bacterial*
Spiroplasma / genetics*

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