Document Detail


Heterogeneity of epithelial and stromal cells of head and neck squamous cell carcinomas in ex vivo chemoresponse.
MedLine Citation:
PMID:  19771432     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Valid prediction of the effectiveness of chemotherapeutic agents in individual head and neck squamous cell carcinoma (HNSCC) is desirable and might be achieved using ex vivo assays.
METHODS: Three biopsies from each of 15 HNSCC were taken, minced and digested by collagenase. The digested HNSCC was added to serial dilutions of either cisplatin (CIS) or docetaxel (DTX), which were prepared under flavin-protecting conditions in ECM-coated microtiterplates. After 72-h incubation, cultures were methanol-fixed and Giemsa-stained. The cutoff concentration (COC; concentration completely suppressing colony formation) for epithelial cells (EC) and stromal cells (SC) was evaluated.
RESULTS: 12/15 HNSCC (80%) were evaluable. Despite significant correlation of COC of CIS in respect of colony formation of EC or SC, no significant differences in response of individual HNSCC specimens were found in the t test for paired samples (p > 0.16). The same applied to DTX. However, EC and SC showed heterogeneity in chemoresponses leading to COC variability of more than one titration step in 44.1% (CIS) and 20% of HNSCC (DTX). No significant correlation between the COC of both cell populations was found in HNSCC specimens.
CONCLUSIONS: The ex vivo chemoresponse of EC and SC of HNSCC must be analyzed separately.
Authors:
Iris-Susanne Horn; Gunnar Wichmann; Christian Mozet; Andreas Dietz; Ralph Dollner; Katrin Tschöp; Andreas Boehm
Publication Detail:
Type:  Journal Article     Date:  2009-09-22
Journal Detail:
Title:  Cancer chemotherapy and pharmacology     Volume:  65     ISSN:  1432-0843     ISO Abbreviation:  Cancer Chemother. Pharmacol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-16     Completed Date:  2010-05-03     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  7806519     Medline TA:  Cancer Chemother Pharmacol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1153-63     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Leipzig, Liebigstrasse 10-14, 04103, Leipzig, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Antineoplastic Agents / pharmacology
Carcinoma, Squamous Cell / pathology
Cell Line, Tumor
Cell Proliferation / drug effects
Cisplatin / pharmacology*
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Epithelial Cells / drug effects*,  pathology
Female
Head and Neck Neoplasms / pathology
Humans
Male
Middle Aged
Stromal Cells / drug effects*,  pathology
Taxoids / pharmacology*
Tumor Cells, Cultured
Tumor Stem Cell Assay
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Taxoids; 15663-27-1/Cisplatin; 15H5577CQD/docetaxel

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