| Heterogeneity of epidermal growth factor binding kinetics on individual cells. | |
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MedLine Citation:
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PMID: 9251825 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Binding of fluorescein-conjugated epidermal growth factor (EGF) to individual A431 cells at 4 degrees C is measured by a quantitative fluorescence imaging technique. After background fluorescence and cell autofluorescence photobleaching corrections, the kinetic data are fit to simple models of one monovalent site and two independent monovalent sites, both of which include a first-order dye photobleaching process. Model simulations and the results from data analysis indicate that the one-monovalent-site model does not describe EGF binding kinetics at the single-cell level, whereas the two-site model is consistent with, but not proved by, the single-cell binding data. In addition, the kinetics of binding of fluorescein-EGF to different cells from the same coverslip often differ significantly from each other, indicating cell-to-cell variations in the binding properties of the EGF receptor. |
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Authors:
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J C Chung; N Sciaky; D J Gross |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biophysical journal Volume: 73 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 1997 Aug |
Date Detail:
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Created Date: 1997-09-29 Completed Date: 1997-09-29 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1089-102 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003-4505, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Binding Sites Cell Membrane / metabolism* Epidermal Growth Factor / metabolism* Fluoresceins Humans Kinetics Microscopy, Fluorescence / methods Models, Chemical Time Factors Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 62229-50-9/Epidermal Growth Factor |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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