Document Detail

Heterogeneity of endothelial cell phenotype within and amongst conduit vessels of the swine vasculature.
MedLine Citation:
PMID:  22542613     Owner:  NLM     Status:  Publisher    
The purpose of this study was to investigate the extent of endothelial cell phenotypic heterogeneity throughout the swine vasculature, with a focus on the conduit vessels of the arterial and venous circulations. We tested the hypothesis that athero-prone arteries exhibit higher expression of markers of inflammation and oxidative stress than do veins and athero-resistant arteries. The study sample included tissues from 79 castrated, male swine. At sacrifice, endothelial cells were mechanically scraped from isolated segments of the thoracic and abdominal aorta, carotid, brachial, femoral, and renal arteries, and each of these vessel's regionally associated vein, as well as the internal mammary (IMA) and right coronary arteries (RCA). Cells were also taken from two regions of the aortic arch contrasted by athero-prone vs. athero-susceptible haemodynamics. Endothelial cell phenotype was assessed by either immunoblotting or RT-PCR for a host of both pro- and anti- atherogenic markers (e.g., eNOS, p67phox, COX-1, SOD-1, etc). Marked heterogeneity across the vasculature was observed in the expression of both pro- and anti- atherogenic markers, at both the protein and transcriptional levels. In particular, the coronary vascular endothelium expressed higher levels of the oxidative stress marker p67phox (p<0.05 vs. other arteries). In addition, differential expression of eNOS and KLF4 was evident between athero-prone and athero-susceptible regions of the aorta, while expression of eNOS, KLF2, KLF4, and COX-1 was lower in both areas of the aortic arch compared to the IMA. Conduit arteries typically expressed higher levels of both pro- and anti- atherogenic markers relative to their associated veins. We show for the first time that endothelial cell phenotype is variable within vessels, across six major vascular territories, and between the arterial and venous circulations. Importantly, even vessel segments with similar vascular architecture (e.g., brachial and carotid arteries) exhibited regional phenotypic heterogeneity; a finding not expected on the basis of local haemodynamic forces alone.
Grant H Simmons; Jaume Padilla; M Harold Laughlin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-27
Journal Detail:
Title:  Experimental physiology     Volume:  -     ISSN:  1469-445X     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002940     Medline TA:  Exp Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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