| Heterogeneity of caprine kappa-casein macropeptide. | |
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MedLine Citation:
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PMID: 11504384 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The heterogeneity of caprine caseinmacropeptide (CMP) was determined by means of treatments with neuraminidase and acid phosphatase and analyses by anion exchange FPLC and reversed-phase (RP)-HPLC, with on-line and off-line electrospray ionization mass spectrometry. The main CMP components were two non-glycosylated and di-phosphorylated forms, as well as two other mono-phosphorylated species, each corresponding to a genetic variant of caprine kappa-casein due to the silent substitution Ile/Val at position 119. Asialo-aglyco mono- and di-phosphorylated forms were found in the ratios 8-14% and 86-92%, respectively. Approximately 36% of caprine CMP was glycosylated. Based on the obtained molecular masses, the occurrence of tri-, di- and monosaccharide-containing di-phosphorylated CMP are reported, assuming that N-acetylgalactosamine, galactose, N-acetyl and N-glycolylneuraminic acids would constitute the main monosaccharides of caprine CMP. CMP microheterogeneity due to the genetic polymorphism was also observed in the glycosylated forms. |
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Authors:
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F J Moreno; I Recio; A Olano; R López-Fandiño |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of dairy research Volume: 68 ISSN: 0022-0299 ISO Abbreviation: J. Dairy Res. Publication Date: 2001 May |
Date Detail:
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Created Date: 2001-08-15 Completed Date: 2001-12-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985125R Medline TA: J Dairy Res Country: England |
Other Details:
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Languages: eng Pagination: 197-208 Citation Subset: IM |
Affiliation:
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Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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metabolism Animals Caseins / chemistry*, metabolism Chromatography, High Pressure Liquid / methods, veterinary Chromatography, Ion Exchange / methods, veterinary Female Glycosylation Goats / physiology Mass Spectrometry / methods, veterinary Milk / chemistry*, metabolism Neuraminidase / metabolism Peptides / metabolism* Phosphorylation Polymorphism, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Caseins; 0/Peptides; EC 3.1.3.2/Acid Phosphatase; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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