Document Detail


Heterogeneity of caprine kappa-casein macropeptide.
MedLine Citation:
PMID:  11504384     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The heterogeneity of caprine caseinmacropeptide (CMP) was determined by means of treatments with neuraminidase and acid phosphatase and analyses by anion exchange FPLC and reversed-phase (RP)-HPLC, with on-line and off-line electrospray ionization mass spectrometry. The main CMP components were two non-glycosylated and di-phosphorylated forms, as well as two other mono-phosphorylated species, each corresponding to a genetic variant of caprine kappa-casein due to the silent substitution Ile/Val at position 119. Asialo-aglyco mono- and di-phosphorylated forms were found in the ratios 8-14% and 86-92%, respectively. Approximately 36% of caprine CMP was glycosylated. Based on the obtained molecular masses, the occurrence of tri-, di- and monosaccharide-containing di-phosphorylated CMP are reported, assuming that N-acetylgalactosamine, galactose, N-acetyl and N-glycolylneuraminic acids would constitute the main monosaccharides of caprine CMP. CMP microheterogeneity due to the genetic polymorphism was also observed in the glycosylated forms.
Authors:
F J Moreno; I Recio; A Olano; R López-Fandiño
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of dairy research     Volume:  68     ISSN:  0022-0299     ISO Abbreviation:  J. Dairy Res.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-08-15     Completed Date:  2001-12-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  197-208     Citation Subset:  IM    
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / metabolism
Animals
Caseins / chemistry*,  metabolism
Chromatography, High Pressure Liquid / methods,  veterinary
Chromatography, Ion Exchange / methods,  veterinary
Female
Glycosylation
Goats / physiology
Mass Spectrometry / methods,  veterinary
Milk / chemistry*,  metabolism
Neuraminidase / metabolism
Peptides / metabolism*
Phosphorylation
Polymorphism, Genetic
Chemical
Reg. No./Substance:
0/Caseins; 0/Peptides; EC 3.1.3.2/Acid Phosphatase; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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