Document Detail


Heteroexpression of the wheat phytochelatin synthase gene (TaPCS1) in rice enhances cadmium sensitivity.
MedLine Citation:
PMID:  23017837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phytochelatin synthase (PCS) (EC 2.3.2.15) catalyzes the final step of phytochelatins (PCs) biosynthesis. PCs are a family of cysteine-rich thiol-reactive and heavy metal-binding peptides that play an important role in sequestration and detoxification of heavy metals in plants. Previous studies have indicated that plants that overexpressed PCS displayed contrasting phenotypes, ranging from enhanced cadmium (Cd) tolerance to Cd hypersensitivity in Arabidopsis thaliana. In this study, the wheat phytochelatin synthase gene, TaPCS1, was heteroexpressed in wild-type rice (Oryza sativa L., cv. Zhonghua 11) to evaluate the relationship between synthesis of PCs and Cd tolerance in rice. Data showed that the heteroexpression of TaPCS1 in rice enhanced Cd sensitivity and significantly increased Cd accumulation in shoots, but not in roots. Additionally, the PCS line exhibited a much higher content of PCs and non-protein thiols (NPTs) in shoots. Prominent changes in NPT composition led to reduced glutathione pool depletion and higher Cd content in cell organelles in shoots, followed by higher oxidative stress, which might result in Cd sensitivity. Therefore, the heteroexpression of TaPCS1 in rice is capable of increasing Cd accumulation in rice shoots and enhancing Cd sensitivity.
Authors:
Feijuan Wang; Zhubing Wang; Cheng Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biochimica et biophysica Sinica     Volume:  44     ISSN:  1745-7270     ISO Abbreviation:  Acta Biochim. Biophys. Sin. (Shanghai)     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2013-02-27     Revised Date:  2013-04-25    
Medline Journal Info:
Nlm Unique ID:  101206716     Medline TA:  Acta Biochim Biophys Sin (Shanghai)     Country:  China    
Other Details:
Languages:  eng     Pagination:  886-93     Citation Subset:  IM    
Affiliation:
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Aminoacyltransferases / genetics*,  metabolism
Cadmium / metabolism,  toxicity*
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Glutathione / metabolism
Glutathione Disulfide / metabolism
Hydrogen Peroxide / metabolism
Oryza sativa / drug effects,  genetics*,  metabolism
Oxidative Stress / drug effects
Plant Roots / drug effects,  genetics,  metabolism
Plant Shoots / drug effects,  genetics,  metabolism
Plants, Genetically Modified
Reverse Transcriptase Polymerase Chain Reaction
Seedling / drug effects,  genetics,  metabolism
Sulfhydryl Compounds / metabolism
Triticum / enzymology,  genetics*
Chemical
Reg. No./Substance:
0/Sulfhydryl Compounds; 27025-41-8/Glutathione Disulfide; 70-18-8/Glutathione; 7440-43-9/Cadmium; 7722-84-1/Hydrogen Peroxide; EC 2.3.2.-/Aminoacyltransferases; EC 2.3.2.15/glutathione gamma-glutamylcysteinyltransferase
Comments/Corrections
Erratum In:
Acta Biochim Biophys Sin (Shanghai). 2013 Mar;45(3):239

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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