| Heteroexpression of the wheat phytochelatin synthase gene (TaPCS1) in rice enhances cadmium sensitivity. | |
| | |
MedLine Citation:
|
PMID: 23017837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Phytochelatin synthase (PCS) (EC 2.3.2.15) catalyzes the final step of phytochelatins (PCs) biosynthesis. PCs are a family of cysteine-rich thiol-reactive and heavy metal-binding peptides that play an important role in sequestration and detoxification of heavy metals in plants. Previous studies have indicated that plants that overexpressed PCS displayed contrasting phenotypes, ranging from enhanced cadmium (Cd) tolerance to Cd hypersensitivity in Arabidopsis thaliana. In this study, the wheat phytochelatin synthase gene, TaPCS1, was heteroexpressed in wild-type rice (Oryza sativa L., cv. Zhonghua 11) to evaluate the relationship between synthesis of PCs and Cd tolerance in rice. Data showed that the heteroexpression of TaPCS1 in rice enhanced Cd sensitivity and significantly increased Cd accumulation in shoots, but not in roots. Additionally, the PCS line exhibited a much higher content of PCs and non-protein thiols (NPTs) in shoots. Prominent changes in NPT composition led to reduced glutathione pool depletion and higher Cd content in cell organelles in shoots, followed by higher oxidative stress, which might result in Cd sensitivity. Therefore, the heteroexpression of TaPCS1 in rice is capable of increasing Cd accumulation in rice shoots and enhancing Cd sensitivity. |
| | |
Authors:
|
Feijuan Wang; Zhubing Wang; Cheng Zhu |
Related Documents
:
|
23212207 - Optimization and/or acclimatization of activated sludge process under heavy metals stress. 21752537 - Gas-particle concentrations of atmospheric polycyclic aromatic hydrocarbons at an urban... 19223587 - Reducing environmental risk by improving n management in intensive chinese agricultural... 12501327 - Effect of six species of white-rot basidiomycetes on the chemical composition and rumen... 15010537 - Identification of the bacterial alarmone guanosine 5'-diphosphate 3'-diphosphate (ppgpp... 3415237 - Efficacy of chemical dosing methods for isolating nontuberculous mycobacteria from wate... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Acta biochimica et biophysica Sinica Volume: 44 ISSN: 1745-7270 ISO Abbreviation: Acta Biochim. Biophys. Sin. (Shanghai) Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-09-28 Completed Date: 2013-02-27 Revised Date: 2013-04-25 |
Medline Journal Info:
|
Nlm Unique ID: 101206716 Medline TA: Acta Biochim Biophys Sin (Shanghai) Country: China |
Other Details:
|
Languages: eng Pagination: 886-93 Citation Subset: IM |
Affiliation:
|
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aminoacyltransferases
/
genetics*,
metabolism Cadmium / metabolism, toxicity* Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Glutathione / metabolism Glutathione Disulfide / metabolism Hydrogen Peroxide / metabolism Oryza sativa / drug effects, genetics*, metabolism Oxidative Stress / drug effects Plant Roots / drug effects, genetics, metabolism Plant Shoots / drug effects, genetics, metabolism Plants, Genetically Modified Reverse Transcriptase Polymerase Chain Reaction Seedling / drug effects, genetics, metabolism Sulfhydryl Compounds / metabolism Triticum / enzymology, genetics* |
| Chemical | |
Reg. No./Substance:
|
0/Sulfhydryl Compounds; 27025-41-8/Glutathione Disulfide; 70-18-8/Glutathione; 7440-43-9/Cadmium; 7722-84-1/Hydrogen Peroxide; EC 2.3.2.-/Aminoacyltransferases; EC 2.3.2.15/glutathione gamma-glutamylcysteinyltransferase |
| Comments/Corrections | |
Erratum In:
|
Acta Biochim Biophys Sin (Shanghai). 2013 Mar;45(3):239 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cloning and primary characterizations of rLcn9, a new member of epididymal lipocalins in rat.
Next Document: The female millipede reproductive system and its dynamical changes, with a partial review.