Document Detail

Heterochromatin and ribosomal genes in Asellus aquaticus (Crust. Isop.).
MedLine Citation:
PMID:  8793199     Owner:  NLM     Status:  MEDLINE    
In the present investigation chromosomal preparations of Asellus aquaticus were sequentially stained with chromomycin A3 to reveal the heterochromatic areas, hybridized in situ with rDNA probes in order to map the ribosomal genes and finally silver stained to check the transcriptional activity of these genes. The results indicate the existence of a substantial correspondence of location and size among the heterochromatic regions and the regions over which the in situ hybridization signals spread. The ribosomal genes, quite independently of their location in the secondary constriction, can be silver stained and thus appear to be transcriptionally active. The ribosomal sequences also hybridize to the entire heterochromatic areas observed on the probable Y chromosome identified in some males of a natural population. These rRNA genes are only rarely transcriptionally active.
R Barzotti; F Pelliccia; A Rocchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  4     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-12-09     Completed Date:  1996-12-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  177-81     Citation Subset:  IM    
Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Rome, Italy.
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MeSH Terms
Chromosome Mapping
Crustacea / genetics*
DNA, Ribosomal / genetics*
Heterochromatin / genetics*,  ultrastructure
In Situ Hybridization, Fluorescence
Nucleolus Organizer Region / ultrastructure*
RNA, Ribosomal / genetics*
Silver Staining
Reg. No./Substance:
0/DNA, Ribosomal; 0/Heterochromatin; 0/RNA, Ribosomal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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