Document Detail


Hesperetin: a potent antioxidant against peroxynitrite.
MedLine Citation:
PMID:  15453641     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peroxynitrite (ONOO-) is a reactive oxidant formed from superoxide (*O2(-)) and nitric oxide (*NO), that can oxidize several cellular components, including essential protein, non-protein thiols, DNA, low-density lipoproteins (LDL), and membrane phospholipids. ONOO- has contributed to the pathogenesis of diseases such as stroke, heart disease, Alzheimer's disease, and atherosclerosis. Because of the lack of endogenous enzymes to thwart ONOO- activation, developing a specific ONOO- scavenger is remarkably important. In this study, the ability of hesperetin (3',5,7-trihydroxy-4-methoxyflavanone) to scavenge ONOO- and to protect cells against ONOO- and ROS was investigated. The data gained show that hesperetin can efficiently scavenge authentic ONOO- . In spectrophotometric analysis, the data revealed that hesperetin led to declined ONOO- -mediated nitration of tyrosine through electron donation. Hesperetin exhibited significant inhibition on the nitration of bovine serum albumin (BSA) by ONOO- in a dose-dependent manner. Hesperetin also manifested cytoprotection from cell damage induced by ONOO- and ROS. The present study suggests that hesperetin is a powerful ONOO- scavenger and promotes cellular defense activity in the protection against ONOO- involved diseases.
Authors:
Ji Young Kim; Kyung Jin Jung; Jae Sue Choi; Hae Young Chung
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical research     Volume:  38     ISSN:  1071-5762     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-09-29     Completed Date:  2005-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  761-9     Citation Subset:  IM    
Affiliation:
College of Pharmacy, Aging Tissue Bank, Pusan National University, Gumjung-gu, Busan 609-735, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / chemistry,  metabolism,  pharmacology*
Cell Line
Free Radical Scavengers / chemistry,  metabolism,  pharmacology
Hesperidin / chemistry,  metabolism,  pharmacology*
Nitric Oxide / chemistry,  metabolism
Peroxynitrous Acid / biosynthesis,  metabolism*
Rats
Reactive Oxygen Species / metabolism
Serum Albumin, Bovine / chemistry,  metabolism
Superoxides / chemistry,  metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Free Radical Scavengers; 0/Reactive Oxygen Species; 0/Serum Albumin, Bovine; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides; 14691-52-2/Peroxynitrous Acid; 520-26-3/Hesperidin; 520-33-2/hesperetin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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