Document Detail


Herpesviruses in abscesses and cellulitis of endodontic origin.
MedLine Citation:
PMID:  19166769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acute apical abscesses and cellulitis are severe endodontic diseases caused by opportunistic bacteria with possible coinfection with latent herpesviruses. The objectives of this study are to identify herpesviruses, including human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), herpes simplex virus-1 (HSV-1), and Varicella zoster virus (VZV) in patients (n = 31) presenting with acute apical abscesses and cellulitis of endodontic origin. Primary and nested polymerase chain reaction (PCR) was conducted using virus-specific primers and DNA isolated from cell-free abscess fluid. From patients exhibiting concurrent spontaneous pain (n = 28), nine abscesses contained HCMV, two abscesses contained EBV, one abscess contained HSV-1, and no abscesses contained VZV. Control PCR using genomic or recombinant templates showed detection limits to a single genomic copy of HCMV, 100 genomic copies for EBV, and 1 to 10 copies for HSV-1 with no cross-amplification between herpesviral DNA targets. Nested PCR was required for detection of herpesviral DNA in the abscess specimens, indicating that these viruses were present in low copy number. Filtration of abscess specimens and virus transfer experiments using human fibroblastic MRC-5 cells confirmed the presence of HCMV particles in several abscess specimens. We conclude that herpesviruses are present but not required for the development of acute apical abscesses and cellulitis of endodontic origin.
Authors:
Vicky Chen; Yanwen Chen; Hong Li; Karla Kent; J Craig Baumgartner; Curtis A Machida
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of endodontics     Volume:  35     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-26     Completed Date:  2009-05-22     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  182-8     Citation Subset:  D    
Affiliation:
Department of Integrative Biosciences, Oregon Health & Science University School of Dentistry, Portland, Oregon 97239, USA.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Adolescent
Adult
Aged
Aged, 80 and over
Alveolar Bone Loss / virology
Cellulitis / virology
Cytomegalovirus / isolation & purification,  pathogenicity
DNA, Viral / analysis
Female
Herpesviridae / genetics,  isolation & purification,  pathogenicity*
Herpesvirus 1, Human / isolation & purification,  pathogenicity
Herpesvirus 4, Human / isolation & purification,  pathogenicity
Humans
Male
Middle Aged
Periapical Abscess / virology
Periapical Diseases / virology*
Polymerase Chain Reaction
Toothache / virology
Virion / isolation & purification
Young Adult
Grant Support
ID/Acronym/Agency:
UL1 RR024140/RR/NCRR NIH HHS; UL1 RR024140/RR/NCRR NIH HHS; UL1 RR024140-02/RR/NCRR NIH HHS; UL1 RR024140-03/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Viral
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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