Document Detail

Herpes simplex virus induces unscheduled DNA synthesis in virus-infected cervical cancer cell lines.
MedLine Citation:
PMID:  1336207     Owner:  NLM     Status:  MEDLINE    
We evaluated herpes-simplex-virus-type-2(HSV2)-induced unscheduled DNA synthesis in virus-infected cervical cancer (HeLa, CaSki, C-33A, and SiHa) cells. HSV2 replication was approximately 100-fold more efficient in the HeLa cells than in less susceptible C-33A and SiHa cells. In dual parameter flow cytometric analysis of bromodeoxyuridine (BrdU) incorporation, HSV2-infected HeLa cells showed a rapid increase in the proportions of DNA-synthesizing G1- and S-phase cells, whereas in C-33A and SiHa cells, the proportions of DNA-synthesizing G1- and early S-phase cells were increased late in the infection. Blocking of HSV2 replication by phosphonoformate inhibited virus-induced changes in HeLa cells, but not in C-33A and SiHa cells. Anti-BrdU antibodies exhibited a coarse globular nuclear staining pattern in the C-33A cells, while the other cells showed speckled and/or fine globular nuclear fluorescence. Anti-ICP8 (HSV-specified major DNA-binding protein) antibodies revealed that, in C-33A cells, ICP8 remained in the cytoplasm, whereas in the other cells, speckled or globular nuclear fluorescence was found. Our results showed that HSV2 induced the unscheduled synthesis of cellular DNA, which was host-cell-dependent, and in virus infected C-33A cells, it may be attributable to both viral and cellular proteins.
P Kulomaa; J Paavonen; M Lehtinen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Research in virology     Volume:  143     ISSN:  0923-2516     ISO Abbreviation:  Res. Virol.     Publication Date:    1992 Sep-Oct
Date Detail:
Created Date:  1993-02-09     Completed Date:  1993-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8907469     Medline TA:  Res Virol     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  351-9     Citation Subset:  IM    
Institute of Biomedical Sciences, University of Tampere, Finland.
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MeSH Terms
Antibodies, Viral
Bromodeoxyuridine / metabolism
DNA, Neoplasm / biosynthesis*
Flow Cytometry
Foscarnet / pharmacology
G1 Phase
Hela Cells
S Phase
Simplexvirus / immunology,  physiology*
Tumor Cells, Cultured
Uterine Cervical Neoplasms / genetics,  microbiology*,  pathology
Viral Proteins / analysis,  immunology
Reg. No./Substance:
0/Antibodies, Viral; 0/DNA, Neoplasm; 0/Viral Proteins; 0/herpes simplex virus type-2 protein ICP8; 4428-95-9/Foscarnet; 59-14-3/Bromodeoxyuridine

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