Document Detail

Herpes simplex virus 1 infected neuronal and skin cells differ in their susceptibility to complement attack.
MedLine Citation:
PMID:  12100729     Owner:  NLM     Status:  MEDLINE    
Herpes simplex virus type 1 (HSV-1) infection in neurons is lifelong and generally asymptomatic. Reactivation of this latent infection results in skin blistering whereas the respective peripheral neurons are rarely affected. Why the neuronal cells are spared while the skin cells are sacrificed is not well understood. In the present study our aim was to study whether neuronal and skin cells differ in their ability to control complement attack during HSV-1 infection. Human embryonal skin (HES) cells and neuronal Paju cells were infected by HSV-1 in vitro. Both types of infected cells activated complement but were initially resistant to membrane attack complex (MAC) deposition. During the first hours of infection the expression of the endogenous complement regulators decay accelerating factor (DAF) and CD59 increased on both HES and Paju cells. By 12 hr the infected HES cells had lost their ability to control complement attack. The expression of DAF and CD59 decreased and the cells became targets for MAC attack. In contrast, complement regulator expression on the Paju cells did not decrease below the initial level and complement C5b-9 deposition was found only on 10% of the Paju cells at 12 hr. The results suggest that HSV-infected neuronal cells are better than skin cells in protecting themselves against complement attack. This may contribute to the persistence of a latent HSV-1 infection in neuronal cells for prolonged periods.
Riina Rautemaa; Tuula Helander; Seppo Meri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  106     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-08     Completed Date:  2002-08-28     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  404-11     Citation Subset:  IM    
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki and the Helsinki University Central Hospital, Finland.
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MeSH Terms
Antigens, CD55 / immunology
Antigens, CD59 / immunology
Cell Culture Techniques
Complement Activation*
Complement Membrane Attack Complex / immunology
Fluorescent Antibody Technique, Indirect
Herpes Simplex / immunology*
Herpesvirus 1, Human / immunology*
Immune Tolerance
Neurons / immunology,  virology*
Skin / immunology,  virology*
Tumor Cells, Cultured
Virus Replication
Reg. No./Substance:
0/Antigens, CD55; 0/Antigens, CD59; 0/Complement Membrane Attack Complex

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