| Herpes simplex keratitis in South India: clinico-virological correlation. | |
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MedLine Citation:
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PMID: 10482477 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: A retrospective cross-section study to analyze the prevalence of herpes simplex virus-induced keratitis (HSK) among 3,000 patients attending a corneal clinic in South India between 1995 and 1997, and to evaluate laboratory techniques for detecting HSK. METHODS: The clinico-virological correlation was studied using herpes simplex virus (HSV) isolation on the Vero cell line, HSV-specific antigen detection by indirect immunofluorescence (IF) microscopy, and serum anti-HSV IgG quantitation, IgM estimation, and tear secretory IgA (sIgA) detection by ELISA. OBSERVATIONS: HSK had a prevalence of 7.8% (234 patients) in this study. A virological correlation could be obtained in 44.4% of the cases that had epithelial manifestations and in 14.8% of the cases that had only stromal disease. In 161 cases where both culture and IF microscopy were used, IF detected 27 cases (26.8%) more than cell culture. The difference in sensitivity between cell culture and IF was found to be statistically significant (McNemar's test, P < .05). An elevation in IgG titer was seen in 17 (30.4%) cases. IgM was detected in only 2 cases of the 62 (3.2%) analyzed. Of the 138 cases analyzed, sIgA was positive in 28 (20.3%) cases. A proved diagnosis could be made in 58% of cases when the specimen was collected during the first week after disease onset, and in only 5% when the time interval increased to 4 weeks. CONCLUSIONS: HSV antigen detection by indirect IF is a rapid and sensitive diagnostic tool for HSK. Tear secretory IgA (sIgA) is a specific marker for acute herpetic keratitis, and the detection of HSV-specific tear sIgA is a valuable adjunct to virus isolation and antigen detection in the laboratory diagnosis of HSK. For a successful diagnosis, the specimen should be collected as soon as possible after HSK onset. |
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Authors:
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N P Pramod; P Rajendran; K A Kannan; S P Thyagarajan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Japanese journal of ophthalmology Volume: 43 ISSN: 0021-5155 ISO Abbreviation: Jpn. J. Ophthalmol. Publication Date: 1999 Jul-Aug |
Date Detail:
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Created Date: 1999-09-29 Completed Date: 1999-09-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0044652 Medline TA: Jpn J Ophthalmol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 303-7 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Animals Antibodies, Viral / analysis Antigens, Viral / analysis Cercopithecus aethiops Child Child, Preschool Cornea / virology* Cross-Sectional Studies Female Fluorescent Antibody Technique, Indirect Herpesvirus 1, Human / immunology, isolation & purification Humans Immunoglobulin A, Secretory / analysis India / epidemiology Infant Keratitis, Herpetic / diagnosis, epidemiology* Male Middle Aged Prevalence Retrospective Studies Tears / immunology Vero Cells / microbiology Virus Cultivation |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/Antigens, Viral; 0/Immunoglobulin A, Secretory |
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