Document Detail


Hereditary spherocytosis and elliptocytosis associated with prosthetic heart valve replacement: rheological study of erythrocyte modifications.
MedLine Citation:
PMID:  19308658     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The implantation of a prosthetic heart valve (HVP) in patients with hereditary spherocytosis (HS) and hereditary elliptocytosis (HE) is rare, and the changes in the structure and deformability of erythrocytes that follow implantation in these patients have been poorly described. In the present study, the erythrocytes in HS and HE patients with mechanical HVP were compared to the erythrocytes in patients with only congenital membrane defects, in terms of biochemical modifications and rheological behaviour. Integral and cytoskeletal erythrocyte membrane proteins were studied, and blood viscosity (shear rate/shear stress ratio), aggregation ratio [eta(1 s(-1))/eta(200 s(-1))], and red cell visco-elasticity were determined. Valve replacement with a mechanical prosthesis worsened anaemia and resulted in a change in haemolysis, from sub-clinical to evident. The rheological investigation of erythrocytes from HS patients confirmed the characteristic increased viscosity and aggregation ratio and the decreased deformability. The rheological behaviour of erythrocytes from patients with HVP showed a decrease in viscosity and an increase in elastic modulus. In these patients, the prosthesis seems to have induced traumatic damage to the erythrocyte membrane, leading to fragmentation and lysis, which in turn modified rheological parameters. The biochemical and rheological investigation allowed us to understand the clinical and haematological pictures of the patients and to describe the role played by different factors in haemolytic anaemia.
Authors:
Patrizia Caprari; Anna Tarzia; Giorgio Mojoli; Paolo Cianciulli; Emilio Mannella; Maria Cristina Martorana
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Publication Detail:
Type:  Journal Article     Date:  2009-03-24
Journal Detail:
Title:  International journal of hematology     Volume:  89     ISSN:  1865-3774     ISO Abbreviation:  Int. J. Hematol.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-09     Completed Date:  2009-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9111627     Medline TA:  Int J Hematol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  285-93     Citation Subset:  IM    
Affiliation:
Department of Haematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy. patrizia.caprari@iss.it
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MeSH Terms
Descriptor/Qualifier:
Adult
Elasticity
Elliptocytosis, Hereditary / complications*
Erythrocytes*
Female
Heart Diseases / complications*,  surgery*
Heart Valve Prosthesis*
Humans
Male
Membrane Proteins / metabolism
Middle Aged
Rheology
Spherocytosis, Hereditary / complications*
Chemical
Reg. No./Substance:
0/Membrane Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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