| Herceptin functionalized microfluidic polydimethylsiloxane devices for the capture of human epidermal growth factor receptor 2 positive circulating breast cancer cells. | |
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MedLine Citation:
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PMID: 21045921 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Building on recent breakthroughs in the field of microfluidic-based capture of rare cancer cells circulating in the blood, the present article reports on the use of Herceptin functionalized PDMS devices designed to efficiently capture from blood cancer cells, overexpressing the tyrosine kinase human epidermal growth factor receptor (HER2). The identification of patients overexpressing HER2 is critical as it typically associates with an aggressive disease course in breast cancer and poor prognosis. Importantly, HER2 positive patients have been found to significantly benefit from Herceptin (Trastuzumab), a humanized monoclonal antibody (MAb) against HER2. Disposable PDMS devices prepared using standard soft lithography were functionalized by the plasma polymerization of an epoxy-containing monomer. The epoxy-rich thin film (AGEpp) thus created could be conjugated with Herceptin either directly or through a polyethylene glycol interlayer. The properties and reactivity toward the monoclonal antibody conjugation of these coatings were determined using x-ray photoelectron spectroscopy; direct conjugation provided a good compromise in reactivity and resistance to biologically nonspecific fouling and was selected. Using the breast cancer cell line SK-BR-3 as a model for cells overexpressing HER2, the immunocapture efficacy of the Herceptin functionalized PDMS was demonstrated in model studies. Validation studies confirmed the ability of the device to efficiently capture (∼80% capture yield) HER2 positive cells from full blood. |
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Authors:
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Benjamin Thierry; Mahaveer Kurkuri; Jun Yan Shi; Lwin Ei Mon Phyo Lwin; Dennis Palms |
Publication Detail:
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Type: Journal Article Date: 2010-09-30 |
Journal Detail:
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Title: Biomicrofluidics Volume: 4 ISSN: 1932-1058 ISO Abbreviation: Biomicrofluidics Publication Date: 2010 |
Date Detail:
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Created Date: 2010-11-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101293825 Medline TA: Biomicrofluidics Country: United States |
Other Details:
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Languages: eng Pagination: 32205 Citation Subset: - |
Affiliation:
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Ian Wark Research Institute, University of South Australia, Mawson Lakes, South Australia 5095, Australia. |
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Descriptor/Qualifier:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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