Document Detail

Herbimycin A attenuates apoptosis during heat stress in rats.
MedLine Citation:
PMID:  12909203     Owner:  NLM     Status:  MEDLINE    
Expression of heat shock proteins (HSPs) as a heat stress response is associated with acquisition of thermotolerance. Herbimycin A is a tyrosine kinase inhibitor that has been shown to induce HSPs. The present study aims to investigate the effects of herbimycin A on thermotolerance in rats subjected to heat stress exposure. Herbimycin A induced hsp70 to peak levels 12 h post-injection in rats without heat stress. No change in hsp70 levels was observed in the vehicle- and saline-treated rats. In rats exposed to heat stress at 45 degrees C for 25 min, 12 h post-treatment, lower peak temperatures were attained in herbimycin A-treated group as compared to the vehicle- and saline-treated groups. Terminal transferase-mediated d-UTP nick end labeling (TUNEL) showed that a significant decrease in apoptosis of hepatocytes in herbimycin A-treated rats as compared to the vehicle- and saline-treated rats. Caspase-3 activation was also lower in herbimycin A-treated rats, compared to the vehicle- and saline-treated rats. The present study has demonstrated that herbimycin A is effective for development of thermotolerance and therefore protects rats from heat stress.
Shankar B Sachidhanandam; Jia Lu; Kerwin S Y Low; Shabbir M Moochhala
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of pharmacology     Volume:  474     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-08-11     Completed Date:  2004-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  121-8     Citation Subset:  IM    
Department of Pharmacology, National University of Singapore, Singapore.
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MeSH Terms
Apoptosis / drug effects*
Blotting, Western
Caspase 3
Caspases / metabolism
Disease Models, Animal
Enzyme Activation
HSP70 Heat-Shock Proteins / biosynthesis
Heat Stress Disorders / enzymology,  metabolism*
In Situ Nick-End Labeling
Lactams, Macrocyclic
Liver / drug effects,  enzymology,  metabolism*
Quinones / pharmacology*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Benzoquinones; 0/HSP70 Heat-Shock Proteins; 0/Lactams, Macrocyclic; 0/Quinones; 70563-58-5/herbimycin; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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