| Herbicide effect on the photodamage process of photosystem II: Fourier transform infrared study. | |
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MedLine Citation:
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PMID: 21718683 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The photodamage process of photosystem II by strong illumination was investigated by examining the herbicide effects on the photoinactivation of redox cofactors. O(2)-evolving photosystem II membranes from spinach in the absence of herbicide and in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and bromoxynil were subjected to strong white-light illumination at 298K, and the illumination-time dependence of the activities of Q(A), the Mn cluster, and P680 were monitored using light-induced Fourier transform infrared (FTIR) difference spectroscopy. The decrease in the Q(A) activity was suppressed and accelerated by DCMU and bromoxynil, respectively, in comparison with the sample without herbicide. The intensity change in the S(2)/S(1) FTIR signal of the Mn cluster exhibited a time course virtually identical to that in the Q(A) signal in all the three samples, suggesting that the loss of the S(1)→S(2) transition was ascribed to the Q(A) inactivation and hence the Mn cluster was inactivated not faster than Q(A). The decrease in the P680 signal was always slower than that of Q(A) keeping the tendency of the herbicide effect. Degradation of the D1 protein occurred after the P680 inactivation. These observations are consistent with the acceptor-side mechanism, in which double reduction of Q(A) triggers the formation of (1)O(2)* to promote further damage to other cofactors and the D1 protein, rather than the recently proposed mechanism that inactivation of the Mn cluster initiates the photodamage. Thus, the results of the present study support the view that the acceptor-side mechanism dominantly occurs in the photodamage to PSII by strong white-light illumination. |
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Authors:
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Issei Idedan; Tatsuya Tomo; Takumi Noguchi |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-27 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: - ISSN: 0006-3002 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-7-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Institute of Materials Science, University of Tsukuba, Tsukuba, Ibaraki, 305-8573, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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